Peptide composition and respective uses

ABSTRACT

The present invention provides a composition that comprises at least one peptide with a sequence length of 6-12 amino acids, where 2-5 of those amino acids are cysteines for the treatment of the hair, including animal and human hair, without the use of chemicals harmful to the hair fiber and consumer health and uses of said compositions in shampoo, lotion, serum, cream, conditioner, foam, elixir, oil, aerosol or mask.

TECHNICAL DOMAIN

The current application corresponds to a composition that comprises at least one peptide, based on keratin and keratin associated proteins, containing 2 to 5 cysteines with the purpose of treatment and cosmetics of animal hair, in preference human hair.

PRIOR ART

Human hair has a significant social role in most of the various world cultures, particularly for female population. Thus, there is a constant desire to improve and change hair characteristics, such as its natural texture.

There are several differences in hair characteristics between different human ethnicities, as well as between individuals of the same ethnicity, such as length, thickness, color and texture.

Hair is composed of approximately 65% to 95% protein. The remaining constituents include water, lipids, pigments and trace elements. The majority of the proteins present in human hair correspond to keratin and keratin-associated proteins.

Human hair fibers structure consists of cuticle, cortex and medulla. The cuticle constitutes about 15% by weight of the hair and consists of overlapping layers of cells, similar to a system of scales, with high content of cysteine. It provides a protective character to the hair fiber. The cortex is the middle region of the hair being responsible for the strength, elasticity and hair color. It is composed of several cell types and represents about 80% of the weight of the hair. The medulla corresponds to a central beam of cells, and is absent in some hairs.

Keratins and mainly keratin-associated proteins have high sulfur content, present in the cysteine amino acid. The presence of sulfur is essential to the hair structure, as it allows the formation of disulfide bonds between amino acids of the polypeptide chains, due to oxidation of cysteine. The existence of these bonds is largely responsible for the structure and texture of the hair.

There are several hair styling methods that involve breakage and reestablishment of disulfide bonds, allowing relaxation and straightening of the hair. However, the most effective methods currently used to modulate hair contain harmful chemicals such as sodium hydroxide, potassium hydroxide, lithium hydroxide, guanidine hydroxide, ammonium thioglycolate or sodium sulfate. These methods can damage the scalp and the hair fiber, leading to its weakening and reducing its tensile strength. Formaldehyde, an extremely toxic chemical, is also used in hair straightening products. Other hair treatments that do not involve so much damage to the hair and the consumer are usually very expensive, time-consuming and/or have low efficacy. Thus there is a constant demand for formulations that efficiently model the hair fiber without damage.

Peptides, proteins, amino acids and its derivatives have also been used in compositions for personal care products, namely hair conditioning and strengthening. For example, the document WO 00/23039 discloses a composition for hair treatment containing intermediate filament proteins, namely artificial keratin. The document EP 0488242 discloses a hair treating agent containing 3% to 10% by weight of cysteine and salts thereof, a polyhydric alcohol or a saccharide containing four to twenty carbon atoms, three or more hydroxyl groups in the molecule and no aldehyde or ketone group.

The current invention is distinguished by the use of peptides, while the other applications refer the use of, respectively, proteins and amino acids in isolation and together with other types of compounds. The peptides in this innovation peptide can penetrate into the human hair in order to improve hair fiber resistance.

The document WO 00/51556 discloses a hair treatment composition that contains four or more discrete amino acids selected from histidine, lysine, methionine, tyrosine, tryptophan or cysteine. This document describes peptides without referring sequences and providing a composition essentially based on histidine, lysine, methionine, tyrosine, tryptophan or cysteine.

The document PT 103484 describes a formulation for cosmetic applications that uses hydrophobic binding domains and/or carbohydrates, in order to enhance its properties and to repair hair damage. The binding domains used are hydrolyzed milk protein, a model of human surfactant protein as well as biologically active and synthetic peptides. The current invention is distinguished by the innovative use of synthetic peptide sequences analogous to keratin proteins instead of surfactant proteins. Furthermore, it does not rely on hydrophobic binding domains and/or carbohydrates, but in other interactions, namely disulfide bonds.

Enzymes have also been used as activating agents for hair treatment, such as in the document WO 00/64405. The document WO 2012/13593 discloses a cosmetic kit for hair conformational change that acts specifically in the disulfide bonds of the hair keratin, through enzyme activating agents and proteolytic enzymes.

As described in the last document there are hair treatments that include actions at the level of the hair disulfide bonds. Below we highlight some examples.

The document WO 97/11672 reports a method for permanent hair processing using tris(2-carboxyethyl)phosphine (TCEP), and other water-soluble tertiary phosphines to break disulfide bonds, whose reaction occurs in acidicic environment. The document U.S. Pat. No. 5,635,170 discloses a composition for permanent shaping of hair based on a keratin reducing agent, which contains N-glycyl-L-cysteine and/or L-cysteinyl-glycine. The pH range of this composition is 6.5 to 9.0. The document WO 2008/081348 refers a method and composition for permanent modulation of hair, through the use of 1% to 30% of N-alkyl-2-mercapto acetamide as a keratin reducing agent. It also contains at least one cationic surfactant for permanently shaping hair and the resulting process. The document WO 2006/001536 describes an agent for permanent hair processing that contains a derivative of mercaptocarboxylic acid, which allows processing and reduction of hair keratin in the acidic and neutral range of the pH. The document US 2010/0272666 discloses a hair cosmetic composition for hair treatment, containing 5 to 50 amino acids, without containing cysteine or its derivatives. Thus, this invention is distinguished by the existence of specific amino acid sequences, which contain cysteine, allowing the formation of disulfide bonds that stabilize and protect the hair fiber.

In a previous article by Fernandes et al. (Fernandes, Lima, Loureiro, Gomes, & Cavaco-Paulo, 2012), it is performed the toxicology evaluation of a peptide sequence for hair care use, containing 13 amino acids with two cysteines in its composition. However, in this article it is not mentioned or suggested that the percentage of cysteine in a peptide sequence may have some effect on the resistance of the hair. Also, in the present innovation, the number of amino acids of each peptide sequence is 6 to 12.

General Description

Thus, the present invention aims to provide a composition for treatment of the hair, including animal and human hair, without the use of chemicals harmful to the hair fiber and consumer health and that does not present the drawbacks found in the state of the art.

The compositions described in the current invention, after prolonged use, provide hair with soft, shiny, undamaged texture and with the desired features. The peptide compositions with a specific number of amino acids and cysteines act synergically providing resistance to strength, toughness and elasticity to the hair. Therefore, the compositions of the current invention are particularly relevant for hair treatment, hair dying, hair perms, etc.

The present application describes a peptide composition for hair treatment, in particular human or animal hair, which comprises at least one peptide with 6-12 amino acids length (namely 6, 7, 8, 9, 10, 11, 12 amino acids), where 2-5 of those amino acids correspond to cysteine, preferably 2, 3, 4 or 5 of those amino acids are cysteines and dermatologically suitable excipients, which penetrates the hair, increasing it resistance and reducing it breakage.

In the embodiment, for improved results, the peptide (or peptides) of the peptide composition for hair care can comprise 10-11 amino acids.

In the embodiment of the peptide composition for hair care treatment, the referred peptides can also contain a percentage of hydrophobic amino acids, not higher than 60%, and preferably less than 41% for better results. Preferably, the composition can also comprise at least one hydrophobic amino acid selected from the following list: phenylalanine, alanine, leucine, methionine, isoleucine, tryptophan, proline, valine or their mixtures.

In yet another embodiment, the amount of cysteine of the peptide composition for hair treatment may vary from 10% to 50% of the total of amino acids of the peptide sequence, preferably 20-30%, and even more preferably 25%.

In an embodiment of the composition, with better results of the peptide (or peptides) of the peptide composition for hair treatment, the sequence of peptide(s) can comprise at least one sequence of the following list with a with a degree of homology greater than or equal to 90%: SEQ.ID NO:1-SEQ.ID NO:1239, preferably with a degree of homology greater than or equal to 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%.

In an embodiment, improved results for the peptide (or peptides) of the peptide composition for hair treatment can comprise at least one of the sequences of the following list with a degree of homology equal or greater than 90%: SEQ.ID NO:5, SEQ.ID NO:75; SEQ.ID NO:94; SEQ.ID NO: 409; SEQ.ID NO:411; SEQ.ID NO:412; SEQ ID. NO:432; SEQ.ID NO:618; SEQ.ID NO:717; SEQ.ID NO:951; SEQ.ID NO:1088; SEQ.ID NO:1131; SEQ.ID NO:1149, preferably with a degree of homology equal or greater than 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%.

In other embodiment, the concentration of the peptide of the peptide composition for hair treatment can vary between 0.001%-20% (w/w), preferably 0.01-5% (w/w).

In yet other embodiment, the peptide composition for hair treatment can comprise at least one excipient, selected from the following list: surfactants, emulsifiers, preservatives, thickeners, organic polymers, humectants, silicones, oils, fragrances, vitamins, buffers.

In another embodiment, the peptide composition for hair treatment can comprise at least one anionic surfactant selected from the following list: alkylbenzene sulfonates, ammonium lauryl sulfate, ammonium lauryl sulfate, ammonium xylenesulfonate, sodium C14-16 olefin sulfonate, sodium cocoyl sarcosinate, sodium laureth sulfate, sodium lauryl sulfate, sodium lauryl sulfoacetate, sodium myreth sulfate, sodium xylenesulfonate, TEA-dodecylbenzenesulfonate, ethyl PEG-15 cocamine sulfate, dioctyl sodium sulfosuccinate, or any mixture thereof.

In an embodiment, the peptide composition for hair treatment can comprise at least one amphoteric surfactant selected from the following list: cocamidopropyl betaine, coco betaine, cocoamphoacetate, cocoamphodipropionate, disodium cocoamphodiacetate, disodium cocoamphodipropionate, lauroamphoacetate, sodium cocoyl isethionate, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one cationic surfactant selected from the following list: quaternary ammonium compounds, behentrimonium chloride, behentrimonium methosulfate, benzalkonium chloride, betrimonium chloride, binnamidopropyltrimonium chloride, cocotrimonium chloride, dicetyldimonium chloride, dicocodimonium chloride, dihydrogenated tallow dimethylammonium chloride, hydrogenated Palm trimethylammonium chloride, laurtrimonium chloride, quaternium-15, quaternium-18 bentonite, quaternium-22 hectonite, stearalkonium chloride, tallowtrimonium chloride, tricetyldimonium chloride, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one non-ionic surfactant selected from the following list: decyl glucoside, laureth-10 (lauryl ether 10), laureth-23, Laureth-4, PEG-10 sorbitan laurate, polysorbate-(20, 21, 40, 60, 61, 65, 80, 81), PPG-1 trideceth-6, sorbitol, steareth-(2, 10, 15, 20), C11-21 pareth-(3-30), C12-20 acid PEG-8 ester, or their mixtures.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one emulsifier selected from the following list: caprylic/capric/diglyceryl succinate, C10-15 pareth-(2,4,6,8) phosphate, C14-16 glycol palmitate, C18-20 glycol isostearate, ceteareth-(4-60), cocamidopropyl lauryl ether, deceth-(3-10), DIPA-hydrogenated cocoate, dipentaerythrityl hydroxystearate, dipentaerythrityl hydroxyisostea rate, dipentaerythrityl hexacaprate/caprylate, dodoxynol-(5,6,7,9,12), nonoxynol-(1-35), octoxynol-(1-70), Octyldodeceth-(2,5,16,20,25), Palm kernel glycerides, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one preservative selected from the following list: butyl paraben, diazolidinyl urea, DMDM hydantoin, ethyl paraben, imidazolidinyl urea, iodopropynyl butylcarbamate, isobutyl paraben, methyl paraben, methylchloroisothiazolinone, methylisothiazolinone, phenoxyethanol, propyl paraben, sodium benzoate, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one thickner selected from the following list: aluminum stearates/isostearates/myristates/laurates/palmitates, glycol distearate, hydrogenated castor oil, hydrogenated castor oil hydroxystearate, hydrogenated castor oil isostearate, hydrogenated castor oil stearate, hydrogenated castor PEG-8 esters, PEG-150 distea rate, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one natural polymer derived selected from the following list: carboxymethyl hydroxyethyl celulose, carboxymethyl hydroxypropyl guar, cellulose, ethyl celulose, hydroxybutyl methylcellulose, hydroxyethylcellulose, hydroxymethylcellulose, lauryl polyglucose, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one humectant selected from the following list: 1,2,6 hexanetriol, dipropylene glycol, glycerin, hexylene glycol, panthenol, phytantriol, propylene glycol, sodium PCA, sorbitol, triethylene glycol, polyglyceryl sorbitol, glucose, fructose, polydextrose, potassium PCA, hydrogenated honey, hyaluronic acid, inositol, hexanediol beeswax, hexanetriol beeswax, hydrolyzed elastin, hydrolyzed collagen, hydrolyzed silk, hydrolyzed keratin, erythritol, capryl glycol, isoceteth-(3-10, 20, 30), isolaureth-(3-10, 20, 30), laneth-(5-50), laureth-(1-30), steareth-(4-20), trideceth-(5-50), or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one cationic polymer selected from the following list: polyquaternium-10, polyquaternium-7, polyquaternium-11m guar hydroxypropyltrimonium chloride, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one silicone selected from the following list: amodimethicone, amodimethicone, trideceth-12, cetrimonium, chloride mixture, behenoxy, dimethicone sparingly, cetearyl methicone, cetyl dimethicone, cyclomethicone, cyclopentasiloxane, dimethicone, dimethicone copolyol, dimethicone copolyol, dimethiconol, hydrolyzed wheat protein hydroxypropyl polysiloxane, stearoxy dimethicone sparingly, stearyl dimethicone, trimethylsilylamodimethicone, lauryl methicone copolyol, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one organic oil selected from the following list: mineral oil, paraffin, petrolatum, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one protein selected from the following list: cocodimonium hydroxypropyl hydrolyzed casein, cocodimonium hydroxypropyl hydrolyzed collagen, cocodimonium hydroxypropyl hydrolyzed hair keratin, cocodimonium hydroxypropyl hydrolyzed keratin, cocodimonium hydroxypropyl hydrolyzed rice protein, cocodimonium hydroxypropyl hydrolyzed silk, cocodimonium hydroxypropyl hydrolyzed soy protein, cocodimonium hydroxypropyl hydrolyzed wheat protein, cocodimonium hydroxypropyl silk amino acids, cocoyl hydrolyzed collagen, cocoyl hydrolyzed keratin, hydrolyzed keratin, hydrolyzed oat flour, hydrolyzed silk, hydrolyzed silk protein, hydrolyzed soy protein, hydrolyzed wheat protein, hydrolyzed wheat protein, keratin, potassium cocoyl hydrolyzed collagen, TEA-cocoyl hydrolyzed collagen, TEA-cocoyl hydrolyzed soy protein, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one vitamin selected from the following list: retinol, retinyl palmitate tocopherol acetate, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one ester emollient selected from the following list: butyl myristate, butyl stearate, C12-15 alkyl benzoate, caprylic/capric triglyceride, cetyl octanoate, cetyl stearate, cetearyl stearate, decyl oleate, dimethyl lauramine isostearate, glyceryl stearate, glyceryl adipate, glyceryl arachidate, glyceryl arachidonate, glyceryl behenate, glyceryl caprate, glyceryl caprylate, glyceryl caprylate/caprate, glyceryl citrate/lactate/linoleate/oleate, glyceryl cocoate, glyceryl diarachidate, glyceryl dibehenate, glyceryl dierucate, glyceryl dihydroxystearate, glyceryl diisopalmitate, glyceryl diisostearate, glyceryl dilaurate, glyceryl dilinoleate, glyceryl dimyristate, glyceryl dioleate, glyceryl dipalmitate, glyceryl dipalmitoleate, glyceryl diricinoleate, glyceryl distearate, glyceryl erucate, glycol stearate, isocetyl stearate, isopropyl myristate, isopropyl palmitate, isopropyl stearate, isostearyl stearate, octyl palmitate, octyl stearate, propylene glycol dicaprylate/dicaprate, sorbitan benzoate, sorbitan caprylate, sorbitan isostearate, Sorbitan laurate, sorbitan tristearate, stearyl stearate, tocopheryl linoleate, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one alkanolamide selected from the following list: acetamide MEA, cocamide DEA, cocamide MEA, lactamide MEA, lauramide DEA, lauramide DEA, propylene glycol, lauramide MEA, lecithinamide DEA, linoleamide DEA, linoleamide MEA, linoleamide MIPA, myristamide DEA, myristamide MEA, myristamide MIPA, oleamide DEA, oleamide DEA, oleamide MEA, oleamide MIPA, soyamide DEA, stearamide MEA, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one amine selected from the following list: behentamidopropyl dimethylamine, cocamidopropyl dimethylamine, isostearamidopropyl dimethylamine, lauramidopropyl dimethylamine, myristamidopropyl dimethylamine, oleamidopropyl dimethylamine, palmitamidopropyl dimethylamine, stearamidopropyl dimethylamine, tallamidopropyl dimethylamine, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one pH adjuster selected from the following list: ascorbic acid, citric acid, sodium hydroxide, triethanolamine, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one salt selected from the following list: calcium chloride, magnesium chloride, magnesium sulfate, potassium chloride, potassium glycol sulfate, sodium chloride, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one aliphatic alcohol selected from the following list: behenyl alcohol, cetearyl alcohol, cetyl alcohol, isocetyl alcohol, isostearyl alcohol, lauryl alcohol, myristyl alcohol, stearyl alcohol, C30-50 alcohols, lanolin alcohol, or any mixture thereof.

In another embodiment, the peptide composition for hair treatment can comprise at least one UV filter/sunscreen selected from the following list: benzophenone-(2, 3, 4, 5, 6, 7, 8, 9, or 10), benzophenone-4, benzyl salicylate, benzylidene camphor sulfonic acid, bornelone, ethyl cinnamate, ethylhexyl methoxycinnamate (octyl methoxycinnamate), octoxynol-40, octoxynol-20, octyl methoxycinnamate, octyl salicylate, oxybenzone, phenyl ketone, PEG-25 PABA, polyacrylamidomethyl benzylidene camphor, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one natural oil selected from the following list: coconut oil, jojoba oil, olive oil, palm Oil, safflower oil, sesame seed oil, shea butter, sweet almond oil, wheat germ oil, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise at least one amine oxide selected from the following list: cocamine oxide, lauramine oxide, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one chelate selected from the following list: diiospropyl oxalate, disodium EDTA, disodium EDTA-copper, HEDTA, oxalic acid, potassium citrate, sodium citrate, dodium oxalate, TEA-EDTA, tetrasodium EDTA, trisodium EDTA, trisodium HEDTA, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one fatty acid selected from the following list: arichidonic acid, capric acid, coconut fatty acid, lauric acid, linoleic acid, linolenic acid, myristic acid, palmitic acid, pantothenic acid, stearic acid, caproic acid, capryleth-(4, 6, 9) carboxylic acid, isostearic acid, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one agent antimicrobial/antibacterial selected from the following list: glyoxal, triclosan, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one PEG-modified material selected from the following list: PEG-150 pentaerythirtyl tetrastearate, PEG-(-2, -3, -4, -6, -8, -12, -20, -32, -50, -150, -175) distearate, PEG-10 castor oil, PEG-10 cocamine, PEG-10 cocoate, PEG-10 coconut oil esters, PEG-10 glyceryl oleate, PEG-10 glyceryl pibsa tallate, PEG-10 glyceryl stearate, PEG-10 hydrogenated lanolin, PEG-10 hydrogenated tallow amine, PEG-10 isolauryl thioether, PEG-10 isostearate, PEG-10 lanolate, PEG-10 lanolin, PEG-10 laurate, PEG-10 oleate, PEG-10 olive glycerides, PEG-10 polyglyceryl-2 laurate, PEG-10 propylene glycol, PEG-10 sorbitan laurate, PEG-10 soya sterol, PEG-10 soyamine, PEG-10 stearamine, PEG-10 stearate, PEG-10 stearyl benzonium chloride, PEG-10 tallate, PEG-10 tallow aminopropylamine, PEG-100, PEG-100 castor oil, PEG-100 hydrogenated castor oil, PEG-100 lanolin, PEG-100 stearate, PEG-40 hydrogenated castor Oil, PEG-60, PEG-55 propylene glycol distearate, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one polymer selected from the following list: carbomer, dodecanedioic acid/cetearyl alcohol/glycol copolymer, hydrogenated C6-14 olefin polymers, hydrogenated ethylene/propylene/styrene copolymer: polyacrylic acid, polymethyl methacrylate: polymer, polyvinyl acetate, polyvinyl alcohol, PPG, PPG-25-laureth-25, PPG-5 pentaerithrityl ether, PPG-75-PEG-300-hexylene glycol, polyvinylpyrrolidone, PVP/VA (polyvinylpyrrolidone/vinyl acetate copolymer), sodium carbomer, TEA-carbomer, poloxamer (100-407), poloxamine, polyacrylamidomethylpropane sulfonic acid, polyethylene terephthalate, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one antistatic agent selected from the following list: apricotamidopropyl ethyldimonium ethosulfate, apricotamidopropyl ethyldimonium lactate, cocamidopropyl ethyldimonium ethosulfate, cocamidopropyl ethyldimonium lactate, lauramidopropyl ethyldimonium ethosulfate, lauramidopropyl ethyldimonium lactate, linoleamidopropyl ethyldimonium ethosulfate, linoleamidopropyl ethyldimonium lactate, myristamidopropyl ethyldimonium ethosulfate, myristamidopropyl ethyldimonium lactate, oleamidopropyl ethyldimonium ethosulfate, oleamidopropyl ethyldimonium lactate, stearamidopropyl ethyldimonium ethosulfate, stearamidopropyl ethyldimonium lactate, or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can comprise at least one alcohol selected from the following list: SD alcohol 40, witch hazel, isopropanol, or any mixture thereof.

In yet other embodiment, the peptide composition for hair treatment can comprise fragrances, oils or any mixture thereof.

In other embodiment, the peptide composition for hair treatment can be used in medicine, veterinary and/or for cosmetics, preferably for the treatment of hair, mainly for animal or human, particularly for treating diseases of the scalp, particularly scalp irritation, alopecia areata, lichen planus, folliculitis keloid of the neck, trichorrhexis nodosa, tricodistrophy, pili torti, tricorrexis invaginata, moniletrix, uncombable hair syndrome.

In other embodiment, the composition may comprise a dye agent linked to the N or C-terminal of the referred peptides.

In yet other embodiment is the use of the described composition for hair coloring.

Other aspect of the embodiment is the use of the described composition as a hair strengthener or as fixer of perms and/or curly hairs.

It is also described in this application shampoo, lotion, serum, cream, conditioner, foam, elixir, oil, aerosol or mask comprising the composition presented in this application.

The present application discloses a composition for hair treatment that comprise, in whole or in part, one or more peptide sequences of 6 to 12 amino acid residues based on keratin and keratin-associated proteins having 2 to 5 cysteine residues, preferably having 3 to 5 residues of cysteine, for treatment and cosmetics of the hair, preferably human hair, chemically pre-treated or not. Thus the presence of cysteine in the peptide sequence (higher than 10%, preferably more than 15%) in combination with a percentage of hydrophobic amino acids ensures that the peptides can have a lasting fixation in the hair, improving the human hair properties such as elasticity and strength.

Surprisingly, the described peptide compositions in which the peptide(s) comprising 2 to 5 cysteines allow penetration of the peptide(s) and enhance the properties of hair, preferably 3-5 cysteines. Thus, described peptide(s) containing 2-5 cysteine in order to allow hair penetration and enrichment of the hair properties, such as elasticity, resistance, reduce eventual hair damage, as well as improve and change hair characteristics.

The peptide compositions described in the present application surprisingly enrich and improve the properties and characteristics of the hair, such as elasticity, strength and appearance, repairing damaged keratinous fiber. Therefore, formulation's high cysteine content is used to improve and/or change its characteristics, such as hair curl or uncurl. The sequence of peptides can have also preferably a percentage of hydrophobic amino acids not exceeding 60%, improving even further the results. Examples of hydrophobic amino acids are phenylalanine, alanine, leucine, methionine, isoleucine, tryptophan, proline, valine, and others.

In the context of the present description, the peptide composition can also be applied to the hair and in particular to the human hair as, but not limited to, aqueous solution or conventional shampoo or conditioner. It can also be used as a lotion, foam, aerosol, gel, mask, and application formulation with or without subsequent rinsing.

The concentration of peptide to be used depends on several features such as the condition of the hair, the origin and the formulation of the hair care product.

DETAILED DESCRIPTION

It should be understood that the detailed description and specific examples are indicative of preferred embodiments of the invention and are provided to be illustrative only. This patent is not limited to those mentioned applications.

The present application describes a composition for hair treatment that comprises different peptides, which are based in the structure of keratin and keratin associated proteins.

The compositions described in the present application allow surprisingly the dermo-cosmetic treatment of animal hair, including human hair, chemically pre-treated or not. The composition described in the present invention, through the use of specific peptides, allows the preparation of keratinous fiber damages, due to the high binding capacity of the keratin peptides, including through disulfide bridges.

The described compositions improve the properties and characteristics of the hair, such as elasticity, resistance and appearance, repairing putative damages of the hair.

The peptides here defined are peptide sequences which bind with a certain affinity to the hair. The peptides used in this invention are composed by 6 to 12 amino acids, and are constituted by a minimum of 2 and a maximum of 5 cysteines, preferably 3-5 cysteines.

The peptide composition for hair treatment described allows a resistance increase due to the presence of the cysteine-rich peptide, which leads to the resistance of the hair even after several rinsing.

Every peptide can be used together or separately, as well as all or part of the peptide sequence in the hair composition. Each peptide sequence contains amino acids with sulfur, specifically cysteine, which interacts with the hair and allows the formation of intermolecular cross-linking, stabilizing the keratinous fiber.

The peptide composition described uses a high content on cysteine in order to enrich the hair properties, such as improve elasticity and resistance, reduce putative damage of the hair, improve and/or change hair characteristics. Regarding the interaction with the keratinous fibers, the cysteine is 10% to 50% of the total amount of amino acids of the peptide sequence. Additionally, the number of amino acids of the peptide sequence is preferable from 6 to 12.

The peptides can be used separately or in combination of two or more peptides. The concentration of the peptide to be used depends on several characteristics, such as hair condition, origin and the formulation of the product for hair treatment. The content of the hair composition of the present invention is as example 1-0.001% (w/w) in mass.

The peptides of the present invention can be prepared by conventional methods of peptide synthesis, well known in the state of the art.

Additionally many companies provide customized services for peptide synthesis.

An embodiment of the current invention describes peptides that link to the hair, and which sequence of amino acids includes cysteines where the sequence is selected from the group between the sequences ID NO:1 to sequence ID NO:1239.

The sequence of the 1239 peptides referred is listed in the table of the FIG. 1.

As example of hair, it was used virgin human hair tresses, acquired from the International Hair Importers and Products, Inc. (New York). The term virgin hair is applied to all the hair that was never subject or was at least 10 years without making any chemical treatment. Several different hair samples such as African, Asian and Caucasian hair are commercially available in several companies, such as the company mentioned above. Optionally, the hair samples can be treated, for example, using hydrogen peroxide to bleach the hair, needed for techniques such as hair dying.

In the context of this invention, the peptides can be applied to the hair, such as the human hair in the form of, but not limited to, aqueous or conventional preparation of shampoo or conditioner. It can also be in the form of lotion, foam, spray, gel, mask, formulation applied with or without subsequent rinsing.

This invention can be prepared by peptide coupling with an agent of these preparations directly or via a spacer.

This coupling interaction can be performed by covalent or non-covalent bonds, such as hydrogen bond, electrostatic interactions, hydrophobic interactions or van der Waals interactions. The spacer can be used to separate the peptide from the preparation agent, ensuring that the agent does not interfere with the peptide linkage to the hair.

The present invention can be understood more clearly and accurately by reading the following examples, which are indicative of preferred embodiments of the invention. They are provided for illustration in greater detail of the present invention, without introducing any limitation and without being limited to those applications.

EXAMPLES OF APPLICATIONS

The examples that are within the scope of the claims represent different embodiments of the invention; all other examples are comparative examples.

Example 1

The present application treats human hair through several commercial formulations with and without the use of the peptides from the sequence ID NO: 5. As The hair was supplied from International Hair Importers and Products, Inc. (New York).

The tests were performed with in human hair after 8 treatments of bleaching, at 50° C. in 0.1 M Na₂CO₃/NaHCO₃ buffer, at pH=9, 10% H₂O₂, for 1 hour.

Several formulations were tested:

-   -   hair serum with 15% PG;     -   hair mask.

The mask used in this application was a basic commercial formulation with water, denaturing alcohol, propylene glycol, ether dicaprylic, cetylstearyl alcohol, behentrimonium chloride, cetyl ester, polysorbate 20, hydrolyzed wheat protein, hydrolyzed wheat starch, benzyl alcohol and fragrance.

The hair serum used in this application was a basic commercial formulation with water, denaturing alcohol, propylene glycol, polysorbate 20, hydrolyzed wheat protein, hydrolyzed wheat starch, crosslinked polymer alkyl acrylate/C10-30, triethanolamine, benzyl alcohol, fragrance.

Each of the formulations was tested with and without the peptide sequence ID NO:5, which contains in the sequence 15% of cysteine. The formulations containing the peptide SEQ ID NO:5 had a concentration of peptide of 0.1 mg/mL, in a ratio 1:1 (v/v).

To demonstrate the effect was also tested:

-   -   a peptide whose sequence does not contain cysteine, with         approximately 41% hydrophobic amino acids;     -   a peptide which contains in it sequence 8% cysteine, with         approximately 58% hydrophobic amino acids.

The hair mask was applied to the hair after 8 bleaching treatments, being left to act for 15 minutes, mimicking the procedure indicated in commercial masks. Posteriorly, the hair was washed. The serum was applied to the hair after 8 bleaching treatments, being left to act for 1 hour at 37° C. Posteriorly, the hair was not washed, as in typical commercial procedures the serum should be applied in dry hair. The hair was also tested after 5 applications.

The peptide from the sequence ID NO: 5 was able to penetrate in the hair fiber for all the formulations.

After the treatment, mechanical tests were performed, using a cell with 2.5 N maximum load and a deformation rate of 1.5 mm/min. Each hair was individually mounted in the tensile jig by means of a paper template with a fixed gauge length of 20 mm.

TABLE 1 Young modulus of virgin hair without treatments and after 8 times bleaching treatments. Hair type Young modulus (MPa) Virgin hair 6579 Hair after 8 time bleaching 5294 Serum(with a 15% cysteine and 50% 7149 hydrophobic amino acids peptide) Serum for comparison(with a 41% 6180 hydrophobic amino acid without cysteine peptide) Serum for comparison 6456 (with a 8% cysteine and 58% hydrophobic amino acid peptide) Serum for comparison (without peptide) 6034

TABLE 2 Young modulus for different types of hair treatment. The peptide in these treatments is the peptide from sequence ID NO: 5. Young modulus Young modulus after 1 after 5 Type of treatment application (MPa) applications (MPa) Serum (with a 15% cysteine 7149 7318 and 50% hydrophobic amino acid peptide) Serum for comparison 6034 6112 (without peptide) Mask (with a 15% cysteine 6175 7075 and 50% hydrophobic amino acid peptide) Mask for comparison(without 5514 5685 peptide)

The formulations which contain the sequence ID NO:5 induce an increase in mechanical resistance of the damaged hair. After 5 applications, the hair treated with the sequence ID NO: 5 maintain the high resistance, having a higher increase in the resistance than without the peptide.

Example 2

This example discloses the treatment of human hair with peptides containing cysteine, and in this case the peptide containing the sequence ID NO: 409, based in the assumption that small peptides are able to penetrate in the hair fiber cuticle.

The hair was supplied from International Hair Importers and Products, Inc. (New York). Hair fibers were pre-treated by bleaching. The formulation was tested in different hair types:

-   -   virgin hair washed, with the cuticle intact and absence of         chemical damages;     -   hair after 8 bleaching treatments, at 50° C. in 0.1 M         Na₂CO₃/NaHCO₃ buffer, at pH=9, 10% H₂O₂, for 1 hour.

The incorporation of the peptides was performed by direct application in the hair surface. The mechanical resistance tests were performed after the treatment of the hair with the peptide.

The measurements of mechanical resistance were performed using a cell with 2.5 N maximum load and a deformation rate of 1.5 mm/min. Each hair was individually mounted in the tensile jig by means of a paper template with a fixed gauge length of 20 mm.

As for the results obtained for the mechanical test showed that compared to the control, i.e., virgin hair without bleaching or peptide treatment (Young modulus: 4142±590 MPa), bleaching reduced the Young modulus (2478±567 MPa), while the treatment with the peptide sequence ID NO: 409 after bleaching increased the Young modulus to higher valued than the virgin hair with no treatment (5649±1022 MPa).

Example 3

This example discloses the treatment of human hair with a composition comprising peptides. In this example, the peptide with the sequence ID NO: 412 was tested. The hair was supplied from International Hair Importers and Products, Inc. (New York).

The formulation was tested in different hair types:

-   -   virgin hair washed, with the cuticle intact and absence of         chemical damages;     -   hair after reduction treatment, at 37° C. in phosphate buffer at         pH=8, with 3M GndHCl and 0.05M DTT for 2 hours.

For the treatment with the peptide SEQ ID NO: 412, concentrations of 0.01% (w/w) were used.

The average of the Young modulus for relaxed hair is 3002 MPa, while for relaxed hair fiber after peptide treatment at 0.01% is 4190 MPa. The Young modulus value for virgin hair without treatment is 5214 MPa.

In the maximum load test, for the relaxed hair fiber, the maximum of resistance were 96 MPa, while for the hair fiber relaxed after peptide treatment 126 MPa and for the virgin hair with no treatment 203 MPa.

Regarding hair stretching, the relaxed hair has an average of 51%, while after treatment with the peptide sequence ID NO: 412, has a stretching of 72%. For virgin hair, the average of hair stretching is 58%.

Therefore, it is evident that the peptides are capable to prevent the hair surface degradation and consequently, the hair treated with these peptides has a longer life span.

Example 4

In order to assess the interactions between the keratin and some peptides, a keratin solution was prepared. This procedure was performed by immersing African hair, acquired from the International Hair Importers and Products, Inc. (New York), in a solution containing 8 M urea, 0.2 M sodium dodecyl sulfate and 0.5 M sodium bisulfite. The mixture was heated to 50° C. for 24 h in a shaker bath. The solution was dialyzed for several days against double-distilled water. The keratin solution was then concentrated using AMICON with a 3 kDa cut-off. The keratin was then conjugated with Alexa Fluor 647 carboxylic acid, succinimidyl ester in DMSO anhydrous 5%.

The reaction was incubated for 1 h 30 min at room temperature and in the dark. The Alexa Fluor 647 that did not link to the keratin solution was separated by centrifugation in AMICON with a 3 kDa cut-off for 1 h at 25° C. and 5000×g.

The keratin was then diluted to 1

g/mL in blocking buffer (3% BSA in tris-buffered saline (TBS) with 0.05% Tween 20). The peptides tested were SEQ.ID NO:179, SEQ.ID NO:75, SEQ.ID NO:432, SEQ.ID NO:951, SEQ.ID NO:1108, SEQ.ID NO:1131 and a peptide containing 13 amino acids, including 2 cysteines (X₃CX₅CX₃), where X represents one of known amino acid residues, with the exception of cysteine residue that is represented by the letter C. This peptide is similar to the one tested in Fernandes et al. (Fernandes, Lima, Loureiro, Gomes, & Cavaco-Paulo, 2012).

Several peptides in a concentration of 15 fmol/mm², were attached to a glass through a hydrophilic linked moiety, and were then incubated with the keratin, marked with Alexa Fluor 647, for 2 hours at 37° C.

After incubation, the glasses were rinsed in successive washing solutions: TBS+0.1% Tween 20 and blocking buffer with 3% BSA in TBS+0.1% Tween 20, for 3 minutes in each solution.

The imaging of the glasses was performed in Agilent G2565CA Microarray Scanner System. Three replicas of the each peptide incubation were performed and analyzed.

TABLE 1 Normalized intensity levels of peptide sequences. Number of Hydrophobic Intensity level amino Cysteine amino acids (average ± Sequence acids content content standard deviation) SEQ. ID 10 20% 50% 0.990 ± 0.014 NO: 179 SEQ. ID 10 30% 60% 1.000 ± 0.000 NO: 75 SEQ. ID 10 30% 40% 1.000 ± 0.000 NO: 432 SEQ. ID 10 40% 30% 1.000 ± 0.000 NO: 951 SEQ. ID 11 46% 18% 1.000 ± 0.000 NO: 1108 SEQ. ID 11 46%  9% 1.000 ± 0.000 NO: 1131 X₃CX₅CX₃ 13 15% 38% 0.184 ± 0.084

The peptides SEQ.ID NO:75, SEQ.ID NO:432, SEQ.ID NO:951, SEQ.ID NO:1108, SEQ.ID NO:1131, with percentage of cysteine ranging from 30% to 46%, such as and percentage of hydrophobic amino acids ranging from 9% to 60% were able to obtain an intensity of 1, indicating a very high affinity to keratin. The peptide SEQ.ID NO:179, with 20% and 50% of cysteine and hydrophobic content, respectively showed an slightly inferior but still very high intensity (0.990±0.014). These peptides were compared with a peptide similar to the one described in Fernandes et al. (Fernandes, Lima, Loureiro, Gomes, & Cavaco-Paulo, 2012) containing 2 cysteines in a 13 amino acids sequence. The reduced percentage of cysteine (15%) and higher number of amino acids in the sequence (13 amino acids) lead to a decrease in the intensity to 0.184±0.084, showing an inferior affinity to keratin. This suggests that the higher number of amino acids difficult the reaction of the peptide with the hair keratins. This inferior affinity to keratin leads to less fixation of the peptides in the hair in posterior treatments and consequently providing less improvements in the recovery of the hair characteristics.

List of Peptide Sequences

The sequences of peptides are described by one letter code of amino acids. The code is as follows:

Amino acid - One Letter Code Histidine - H Arginine - R Lysine - K Isoleucine - I Phenylalanine - F Leucine - L Tryptophan - W Alanine - A Methionine - M Proline - P Valine - V Cysteine - C Asparagine - N Glycine - G Serine - S Glutamine - Q Tyrosine - Y Threonine - T Aspartic acid - D Glutamic acid - E SEQ. ID NO: 1 APCAPRPSCG SEQ. ID NO: 2 EACVPSVPCP SEQ. ID NO: 3 ESCGTASGCA SEQ. ID NO: 4 GLCAGTSACL SEQ. ID NO: 5 GVCGPSPPCI SEQ. ID NO: 6 HGCTLPGACN SEQ. ID NO: 7 HSCTLPGACN SEQ. ID NO: 8 KDCLQNSLCE SEQ. ID NO: 9 LPCLPAASCG SEQ. ID NO: 10 LPCYFTGSCN SEQ. ID NO: 11 NFCLPSLSCR SEQ. ID NO: 12 NPCATTNACD SEQ. ID NO: 13 NPCATTNACE SEQ. ID NO: 14 NPCATTNACS SEQ. ID NO: 15 NPCGLRARCG SEQ. ID NO: 16 NPCGPRSRCG SEQ. ID NO: 17 NPCSTPASCT SEQ. ID NO: 18 NPCSTSPSCV SEQ. ID NO: 19 PACTSSSPCS SEQ. ID NO: 20 SKCHESTVCP SEQ. ID NO: 21 SPCVPRTVCV SEQ. ID NO: 22 SSCSVETACL SEQ. ID NO: 23 SVCSSGVNCR SEQ. ID NO: 24 TACPLPGTCH SEQ. ID NO: 25 TNCSPRPICV SEQ. ID NO: 26 TSCVPPAPCT SEQ. ID NO: 27 TTCTSSNTCE SEQ. ID NO: 28 VPCVPSVPCT SEQ. ID NO: 29 ATCGPSACIT SEQ. ID NO: 30 GPCISNPCGL SEQ. ID NO: 31 GPCLSNPCTS SEQ. ID NO: 32 GSCVTNPCGP SEQ. ID NO: 33 LTCFSITCSS SEQ. ID NO: 34 NPCSTPSCTT SEQ. ID NO: 35 PSCVTAPCAP SEQ. ID NO: 36 SDCSSTHCSP SEQ. ID NO: 37 SLCLPPTCHT SEQ. ID NO: 38 SLCNLGSCGP SEQ. ID NO: 39 SPCLVGNCAW SEQ. ID NO: 40 TACLPGTCAT SEQ. ID NO: 41 TSCLPALCLP SEQ. ID NO: 42 TSCSSRPCVP SEQ. ID NO: 43 TTCGGGSCGV SEQ. ID NO: 44 VNCRPELCLG SEQ. ID NO: 45 YVCQPMACLP SEQ. ID NO: 46 AFSCISACGP SEQ. ID NO: 47 GSVCSAPCNG SEQ. ID NO: 48 GVVCGDLCAS SEQ. ID NO: 49 GVVCGDLCVS SEQ. ID NO: 50 LTGCLLPCYF SEQ. ID NO: 51 NEDCKLPCNP SEQ. ID NO: 52 NFSCVSACGP SEQ. ID NO: 53 PPTCHTACPL SEQ. ID NO: 54 PQPCATACKP SEQ. ID NO: 55 SEDCKLPCNP SEQ. ID NO: 56 SLGCRTSCSS SEQ. ID NO: 57 SLSCRTSCSS SEQ. ID NO: 58 SSSCPLGCTM SEQ. ID NO: 59 TGSCNSPCLV SEQ. ID NO: 60 TSSCPLGCTM SEQ. ID NO: 61 VGSCGSSCRK SEQ. ID NO: 62 VGVCGGSCKR SEQ. ID NO: 63 VSNCNWFCEG SEQ. ID NO: 64 ACGPRPGRCC SEQ. ID NO: 65 ACGPRPSRCC SEQ. ID NO: 66 CAPRPSCGPC SEQ. ID NO: 67 CEPCSAYVIC SEQ. ID NO: 68 CGLRARCGPC SEQ. ID NO: 69 CGPRPGRCCI SEQ. ID NO: 70 CGPRPSRCCI SEQ. ID NO: 71 CGPRSRCGPC SEQ. ID NO: 72 CGTSQKGCCN SEQ. ID NO: 73 CHGCTLPGAC SEQ. ID NO: 74 CHSCTLPGAC SEQ. ID NO: 75 CLPCLPAASC SEQ. ID NO: 76 CLPPTCHTAC SEQ. ID NO: 77 CLSNPCTSCV SEQ. ID NO: 78 CLVGNCAWCE SEQ. ID NO: 79 CNPCSTPASC SEQ. ID NO: 80 CNPCSTPSCT SEQ. ID NO: 81 CNPCSTSPSC SEQ. ID NO: 82 CNSPCLVGNC SEQ. ID NO: 83 CRTSCSSRPC SEQ. ID NO: 84 CSLKEHCSAC SEQ. ID NO: 85 CSPRPICVPC SEQ. ID NO: 86 CSSTMSYSCC SEQ. ID NO: 87 CSTPASCTSC SEQ. ID NO: 88 CSTPSCTTCV SEQ. ID NO: 89 CTSCVPPAPC SEQ. ID NO: 90 CTSSNTCEPC SEQ. ID NO: 91 CVPPAPCTPC SEQ. ID NO: 92 CVPPSCHGCT SEQ. ID NO: 93 CVPPSCHSCT SEQ. ID NO: 94 DCKLPCNPCA SEQ. ID NO: 95 DCKLPCNPCS SEQ. ID NO: 96 PCGTSQKGCC SEQ. ID NO: 97 PCLSNPCTSC SEQ. ID NO: 98 PCLVGNCAWC SEQ. ID NO: 99 PCNPCSTPSC SEQ. ID NO: 100 PCSTPSCTTC SEQ. ID NO: 101 PCTTCGPTCG SEQ. ID NO: 102 PCVPPSCHGC SEQ. ID NO: 103 PCVPPSCHSC SEQ. ID NO: 104 SCCLPSLGCR SEQ. ID NO: 105 SCSEELQCCQ SEQ. ID NO: 106 SCSPCSTTCT SEQ. ID NO: 107 ASCSTSGTCG SEQ. ID NO: 108 ASCYIPVGCQ SEQ. ID NO: 109 ASCYVPVSCQ SEQ. ID NO: 110 AVCTLPSSCQ SEQ. ID NO: 111 DLCPTSVSCG SEQ. ID NO: 112 EICWEPTSCQ SEQ. ID NO: 113 ETCGEPTSCQ SEQ. ID NO: 114 ETCNETTSCQ SEQ. ID NO: 115 ETCWRPNSCQ SEQ. ID NO: 116 GYCGYRPFCF SEQ. ID NO: 117 KTCWEPASCQ SEQ. ID NO: 118 KTCWEPTSCQ SEQ. ID NO: 119 LDCVDTTPCK SEQ. ID NO: 120 LGCGYGSFCG SEQ. ID NO: 121 NSCGYGSGCG SEQ. ID NO: 122 NYCPSNTMCE SEQ. ID NO: 123 PACVTSYSCR SEQ. ID NO: 124 PDCHVEGTCL SEQ. ID NO: 125 PDCRVEGTCL SEQ. ID NO: 126 PICSEPSPCS SEQ. ID NO: 127 PICYIFKPCQ SEQ. ID NO: 128 PLCYISNSCQ SEQ. ID NO: 129 PPCGQPTPCS SEQ. ID NO: 130 PPCHIPQPCV SEQ. ID NO: 131 PSCGRLASCG SEQ. ID NO: 132 PSCSESSICQ SEQ. ID NO: 133 PSCSEVTSCP SEQ. ID NO: 134 PSCSTSGTCG SEQ. ID NO: 135 PSCSVSSGCQ SEQ. ID NO: 136 PSCTESDSCK SEQ. ID NO: 137 PSCYQTSSCG SEQ. ID NO: 138 PTCFLLNSCQ SEQ. ID NO: 139 PTCSVTSSCQ SEQ. ID NO: 140 PTCWLLNNCH SEQ. ID NO: 141 PTCYQRTSCV SEQ. ID NO: 142 PTCYRRTSCV SEQ. ID NO: 143 PTCYVVKRCP SEQ. ID NO: 144 PVCFEATICE SEQ. ID NO: 145 PVCFEATVCE SEQ. ID NO: 146 PVCSRPASCS SEQ. ID NO: 147 PVCSWVPACS SEQ. ID NO: 148 QTCNESSYCL SEQ. ID NO: 149 QTCWEPTSCQ SEQ. ID NO: 150 SFCRLGYGCG SEQ. ID NO: 151 SFCRRGSGCG SEQ. ID NO: 152 SLCGYGYGCG SEQ. ID NO: 153 SLCSTEVSCG SEQ. ID NO: 154 SNCFGQLNCL SEQ. ID NO: 155 SPCGQPTPCS SEQ. ID NO: 156 SSCDQSSSCA SEQ. ID NO: 157 SSCGQSSSCA SEQ. ID NO: 158 SVCPEPVSCP SEQ. ID NO: 159 TFCSFDKSCR SEQ. ID NO: 160 TICSSDKSCR SEQ. ID NO: 161 TLCVESSPCH SEQ. ID NO: 162 TPCYQQSSCQ SEQ. ID NO: 163 VICSRQTTCI SEQ. ID NO: 164 YGCGYGSGCG SEQ. ID NO: 165 YGCGYGSGCR SEQ. ID NO: 166 YGCIHSTHCG SEQ. ID NO: 167 AACEPSACQS SEQ. ID NO: 168 AACEPSPCQS SEQ. ID NO: 169 AACTMSVCSS SEQ. ID NO: 170 ADCLGGICLP SEQ. ID NO: 171 ALCLPSSCHS SEQ. ID NO: 172 ALCSPSTCQL SEQ. ID NO: 173 APCLALVCAP SEQ. ID NO: 174 APCLSLVCTP SEQ. ID NO: 175 APCLTLVCTP SEQ. ID NO: 176 APCVALLCRP SEQ. ID NO: 177 ASCGSLLCRP SEQ. ID NO: 178 ASCLSFLCRP SEQ. ID NO: 179 ASCVSLLCRP SEQ. ID NO: 180 AVCEPSPCQS SEQ. ID NO: 181 AVCLPVSCQS SEQ. ID NO: 182 AVCVPVRCQS SEQ. ID NO: 183 AVCVPVSCQS SEQ. ID NO: 184 DLCSPSTCQL SEQ. ID NO: 185 DSCGSSSCGP SEQ. ID NO: 186 DSCVQSNCFP SEQ. ID NO: 187 FNCSTRNCSS SEQ. ID NO: 188 GGCGSYGCSQ SEQ. ID NO: 189 GSCGFGSCYG SEQ. ID NO: 190 GSCSSRKCFS SEQ. ID NO: 191 GVCLPSTCPH SEQ. ID NO: 192 HSCEGYLCYS SEQ. ID NO: 193 IVCAAPSCQS SEQ. ID NO: 194 KTCSTTGCDP SEQ. ID NO: 195 LACVSQPCQS SEQ. ID NO: 196 LGCGYGGCGY SEQ. ID NO: 197 LSCGSRSCSS SEQ. ID NO: 198 LVCTPVSCVS SEQ. ID NO: 199 NGCQETYCEP SEQ. ID NO: 200 NSCRSLSCGS SEQ. ID NO: 201 PACVISTCPR SEQ. ID NO: 202 PGCLNQSCGS SEQ. ID NO: 203 PPCGTAPCLT SEQ. ID NO: 204 PPCTTALCRP SEQ. ID NO: 205 PPCYLVSCTP SEQ. ID NO: 206 PRCTRPICEP SEQ. ID NO: 207 PSCPVSSCAQ SEQ. ID NO: 208 PSCQPSVCVP SEQ. ID NO: 209 PSCSVSNCYQ SEQ. ID NO: 210 PSCSVSSCAQ SEQ. ID NO: 211 PSCTSVLCRP SEQ. ID NO: 212 PTCKSPSCEP SEQ. ID NO: 213 PTCVISSCPR SEQ. ID NO: 214 PTCVISTCPR SEQ. ID NO: 215 PTCYQTICFR SEQ. ID NO: 216 PVCGGVSCHT SEQ. ID NO: 217 PVCGRVSCHT SEQ. ID NO: 218 PVCNKPVCFV SEQ. ID NO: 219 PVCPTPTCSV SEQ. ID NO: 220 PVCRSTYCVP SEQ. ID NO: 221 PVCSKSVCYV SEQ. ID NO: 222 PVCSRPACYS SEQ. ID NO: 223 PVCYVPTCSE SEQ. ID NO: 224 QFCLSKSCQP SEQ. ID NO: 225 RPCERTACQS SEQ. ID NO: 226 RSCQTSFCGF SEQ. ID NO: 227 RSCSSLGCGS SEQ. ID NO: 228 RSCYSVGCGS SEQ. ID NO: 229 RVCLPGSCDS SEQ. ID NO: 230 SFCGFPSCST SEQ. ID NO: 231 SFCGYPSCST SEQ. ID NO: 232 SGCDPASCQP SEQ. ID NO: 233 SGCGGSGCGG SEQ. ID NO: 234 SGCQPSSCLA SEQ. ID NO: 235 SHCQPPHCQL SEQ. ID NO: 236 SICQPATCVA SEQ. ID NO: 237 SLCVPVSCRP SEQ. ID NO: 238 SNCLPTSCQP SEQ. ID NO: 239 SPCLVSSCQP SEQ. ID NO: 240 SPCQQSSCQE SEQ. ID NO: 241 SPCQQSYCVP SEQ. ID NO: 242 SPCSPAVCVS SEQ. ID NO: 243 SRCQQPSCQP SEQ. ID NO: 244 SRCYRPHCGQ SEQ. ID NO: 245 SSCAPIYCRR SEQ. ID NO: 246 SSCAPVYCRR SEQ. ID NO: 247 SSCGKGGCGS SEQ. ID NO: 248 SSCGKRGCGS SEQ. ID NO: 249 SSCLPVSCRP SEQ. ID NO: 250 SSCQPAYCTS SEQ. ID NO: 251 SSCQPSYCRQ SEQ. ID NO: 252 SSCQPVVCEP SEQ. ID NO: 253 SSCTAVVCRP SEQ. ID NO: 254 SSCYQPFCRS SEQ. ID NO: 255 SSCYRPICGS SEQ. ID NO: 256 SSCYRPTCGS SEQ. ID NO: 257 SVCMSGSCQA SEQ. ID NO: 258 SVCSDQGCDQ SEQ. ID NO: 259 SVCSDQGCGL SEQ. ID NO: 260 SVCSDQGCGQ SEQ. ID NO: 261 SVCSDQGCSQ SEQ. ID NO: 262 SVCSDQSCGQ SEQ. ID NO: 263 SVCSHQGCGQ SEQ. ID NO: 264 SVCSHQGCGR SEQ. ID NO: 265 SVCVPVSCRP SEQ. ID NO: 266 SYCRQASCVS SEQ. ID NO: 267 TACEPSACQS SEQ. ID NO: 268 TICTASPCQP SEQ. ID NO: 269 TSCPETSCLP SEQ. ID NO: 270 TSCQMTNCEQ SEQ. ID NO: 271 TSCQPVHCET SEQ. ID NO: 272 TSCQPVLCKS SEQ. ID NO: 273 TSCQPVLCVP SEQ. ID NO: 274 TSCVGFVCQP SEQ. ID NO: 275 TSCVSNPCQV SEQ. ID NO: 276 TTCFQPTCVS SEQ. ID NO: 277 TTCFQPTCVT SEQ. ID NO: 278 TTCFQPTCVY SEQ. ID NO: 279 TTCISNPCST SEQ. ID NO: 280 TWCQGSSCQP SEQ. ID NO: 281 VGCQSSVCVP SEQ. ID NO: 282 VPCQPSTCVF SEQ. ID NO: 283 VSCEPSPCQS SEQ. ID NO: 284 VSCGGPICLP SEQ. ID NO: 285 VSCKPVLCVA SEQ. ID NO: 286 VSCPSTSCRP SEQ. ID NO: 287 VSCQSSVCMP SEQ. ID NO: 288 VSCTRIVCVA SEQ. ID NO: 289 VTCEPSPCQS SEQ. ID NO: 290 VTCQTTVCRP SEQ. ID NO: 291 YGCGYEGCRY SEQ. ID NO: 292 AGSCQPSCSE SEQ. ID NO: 293 ALLCRPLCGV SEQ. ID NO: 294 ALVCEPVCLR SEQ. ID NO: 295 ATICEPSCSV SEQ. ID NO: 296 ATTCEPSCSV SEQ. ID NO: 297 ATVCEPSCSV SEQ. ID NO: 298 EGTCLPPCYL SEQ. ID NO: 299 FSTCRPSCSG SEQ. ID NO: 300 GFVCQPMCSH SEQ. ID NO: 301 GLDCGYGCGY SEQ. ID NO: 302 GLGCGYGCGY SEQ. ID NO: 303 GLGCSYGCGH SEQ. ID NO: 304 GLGCSYGCGL SEQ. ID NO: 305 GSGCGYGCGY SEQ. ID NO: 306 GTGCGYGCGY SEQ. ID NO: 307 GVSCHTTCYR SEQ. ID NO: 308 GYACNFPCSY SEQ. ID NO: 309 GYGCGYGCGF SEQ. ID NO: 310 HSPCQASCYV SEQ. ID NO: 311 HTSCSPACQP SEQ. ID NO: 312 HTSCSSGCQP SEQ. ID NO: 313 IRWCHPDCHV SEQ. ID NO: 314 IRWCRPDCRV SEQ. ID NO: 315 ISSCGTGCGI SEQ. ID NO: 316 KGGCGSGCGG SEQ. ID NO: 317 KGGCGSSCSQ SEQ. ID NO: 318 LVTCQDSCGS SEQ. ID NO: 319 LVTCQESCQP SEQ. ID NO: 320 MSICSSACTD SEQ. ID NO: 321 MSICSSACTN SEQ. ID NO: 322 MSVCSSACSD SEQ. ID NO: 323 PAICEPSCSV SEQ. ID NO: 324 PASCQKSCYR SEQ. ID NO: 325 PIYCRRTCYH SEQ. ID NO: 326 PNSCQTLCVE SEQ. ID NO: 327 PQPCVPTCFL SEQ. ID NO: 328 PSACQSGCTS SEQ. ID NO: 329 PSPCEPSCSE SEQ. ID NO: 330 PSPCQASCYI SEQ. ID NO: 331 PSPCQSGCIS SEQ. ID NO: 332 PSPCQSGCTD SEQ. ID NO: 333 PSPCQSGCTS SEQ. ID NO: 334 PTACQPTCYQ SEQ. ID NO: 335 PTACQPTCYR SEQ. ID NO: 336 PTPCSTTCRT SEQ. ID NO: 337 PTSCQKSCYR SEQ. ID NO: 338 PTSCQPSCES SEQ. ID NO: 339 PTSCQTSCTL SEQ. ID NO: 340 PVICEPSCSV SEQ. ID NO: 341 PVSCVPVCSG SEQ. ID NO: 342 PVTCVPRCTR SEQ. ID NO: 343 PVYCRRTCYH SEQ. ID NO: 344 PVYCRRTCYY SEQ. ID NO: 345 PVYCVPVCSG SEQ. ID NO: 346 QPGCESPCEP SEQ. ID NO: 347 QQSCVSSCRR SEQ. ID NO: 348 QTSCGSSCGQ SEQ. ID NO: 349 QTTCHPSCGM SEQ. ID NO: 350 QTTCRPSCGV SEQ. ID NO: 351 RGGCGSGCGG SEQ. ID NO: 352 RLACYSLCSG SEQ. ID NO: 353 RPACYRPCYS SEQ. ID NO: 354 RPFCFRRCYS SEQ. ID NO: 355 RPICRPICSG SEQ. ID NO: 356 RPLCYRRCYS SEQ. ID NO: 357 RSPCQASCYV SEQ. ID NO: 358 RVSCHTTCYR SEQ. ID NO: 359 SAICRPTCPR SEQ. ID NO: 360 SDSCKRDCKK SEQ. ID NO: 361 SEGCGSGCGG SEQ. ID NO: 362 SFLCRPACSR SEQ. ID NO: 363 SGGCGSGCGG SEQ. ID NO: 364 SGGCGSSCGG SEQ. ID NO: 365 SGSCQAACGQ SEQ. ID NO: 366 SLLCHPVCKS SEQ. ID NO: 367 SLLCHPVCRS SEQ. ID NO: 368 SLLCRPACSP SEQ. ID NO: 369 SLLCRPACSR SEQ. ID NO: 370 SLLCRPICRP SEQ. ID NO: 371 SLLCRPMCSR SEQ. ID NO: 372 SLLCRPTCSR SEQ. ID NO: 373 SLLCRPVCQP SEQ. ID NO: 374 SLLCRPVCRP SEQ. ID NO: 375 SLLCRPVCRS SEQ. ID NO: 376 SLLCRPVCSR SEQ. ID NO: 377 SNPCQVTCSR SEQ. ID NO: 378 SRGCGSGCGG SEQ. ID NO: 379 SRSCQSPCYR SEQ. ID NO: 380 SRSCQSSCYR SEQ. ID NO: 381 SSGCGYGCGY SEQ. ID NO: 382 SSGCPMACPG SEQ. ID NO: 383 SSICQPICSE SEQ. ID NO: 384 SSPCHTSCYY SEQ. ID NO: 385 SSPCQPTCYV SEQ. ID NO: 386 SSPCQQSCYV SEQ. ID NO: 387 SSPCQTSCYR SEQ. ID NO: 388 SSSCQQSCRV SEQ. ID NO: 389 STVCQPACGV SEQ. ID NO: 390 TDNCQETCGE SEQ. ID NO: 391 TQPCYEPCLP SEQ. ID NO: 392 TSSCGTGCGI SEQ. ID NO: 393 TSSCQPSCGR SEQ. ID NO: 394 TSSCTTPCYQ SEQ. ID NO: 395 TSVCLPGCLN SEQ. ID NO: 396 TTVCLPGCLN SEQ. ID NO: 397 VANCQAPCST SEQ. ID NO: 398 VDDCPESCWP SEQ. ID NO: 399 VKRCPSVCPE SEQ. ID NO: 400 VSSCQPSCSE SEQ. ID NO: 401 YEGCRYGCGH SEQ. ID NO: 402 YGRCRHGCHS SEQ. ID NO: 403 YGYCRPSCYG SEQ. ID NO: 404 YRDCQKTCWE SEQ. ID NO: 405 YRGCQEICWE SEQ. ID NO: 406 YRGCQETCWR SEQ. ID NO: 407 YRGCQQTCWE SEQ. ID NO: 408 YRSCRPSCYG SEQ. ID NO: 409 GGVCGPSPPC SEQ. ID NO: 410 GVCGPSPPCI SEQ. ID NO: 411 VCGPSPPCIT SEQ. ID NO: 412 CGPSPPCITT SEQ. ID NO: 413 CAPIYCRRTC SEQ. ID NO: 414 CAPSPCQASC SEQ. ID NO: 415 CAPSPCQPAC SEQ. ID NO: 416 CAPVYCRRTC SEQ. ID NO: 417 CASSPCQQAC SEQ. ID NO: 418 CASSSCQPAC SEQ. ID NO: 419 CASSSCQQSC SEQ. ID NO: 420 CCGNFSSHSC SEQ. ID NO: 421 CCGYGGLGCG SEQ. ID NO: 422 CCNYYGNSCG SEQ. ID NO: 423 CCNYYRNSCG SEQ. ID NO: 424 CCSRNFSSCS SEQ. ID NO: 425 CDAGSCQPSC SEQ. ID NO: 426 CDPCSLQEGC SEQ. ID NO: 427 CDPSPCEPSC SEQ. ID NO: 428 CDPVICEPSC SEQ. ID NO: 429 CDQGLCQETC SEQ. ID NO: 430 CEATTCEPSC SEQ. ID NO: 431 CELPCGTPSC SEQ. ID NO: 432 CEPAICEPSC SEQ. ID NO: 433 CEPPCGTAPC SEQ. ID NO: 434 CEPPCSAPSC SEQ. ID NO: 435 CEPRSCASSC SEQ. ID NO: 436 CEPSACQSGC SEQ. ID NO: 437 CEPSCSVSNC SEQ. ID NO: 438 CEPSCSVSSC SEQ. ID NO: 439 CEPSPCQSGC SEQ. ID NO: 440 CEPTACQPTC SEQ. ID NO: 441 CEPTSCQTSC SEQ. ID NO: 442 CEPVCLRPVC SEQ. ID NO: 443 CETSSCQPRC SEQ. ID NO: 444 CETTCFQPTC SEQ. ID NO: 445 CFQPTCVSSC SEQ. ID NO: 446 CFQPTCVTSC SEQ. ID NO: 447 CFQPTCVYSC SEQ. ID NO: 448 CGCGFRRLGC SEQ. ID NO: 449 CGCGYRGLDC SEQ. ID NO: 450 CGCNGYYGCY SEQ. ID NO: 451 CGFGSCYGCG SEQ. ID NO: 452 CGGSGCGGSC SEQ. ID NO: 453 CGGSGSSCCV SEQ. ID NO: 454 CGGVSCHTTC SEQ. ID NO: 455 CGKGGCGSCG SEQ. ID NO: 456 CGKRGCGSCG SEQ. ID NO: 457 CGQDLCQETC SEQ. ID NO: 458 CGQTSCGSSC SEQ. ID NO: 459 CGQVLCQETC SEQ. ID NO: 460 CGRDLCQETC SEQ. ID NO: 461 CGRVSCHTTC SEQ. ID NO: 462 CGSCGFGSCY SEQ. ID NO: 463 CGSCGGSKGC SEQ. ID NO: 464 CGSGCGVPVC SEQ. ID NO: 465 CGSLLCRPTC SEQ. ID NO: 466 CGSRCYVPVC SEQ. ID NO: 467 CGSSSCGPQC SEQ. ID NO: 468 CGSVCSDQGC SEQ. ID NO: 469 CGSVCSDQSC SEQ. ID NO: 470 CGSVCSHQGC SEQ. ID NO: 471 CGSYGCSQCS SEQ. ID NO: 472 CGVCLPSTCP SEQ. ID NO: 473 CGYEGCRYGC SEQ. ID NO: 474 CGYGCGYGCG SEQ. ID NO: 475 CGYGGCGYGC SEQ. ID NO: 476 CGYGSFCGCG SEQ. ID NO: 477 CGYGSGCGCG SEQ. ID NO: 478 CHPSCGMSSC SEQ. ID NO: 479 CHPSCSISSC SEQ. ID NO: 480 CHPTCYQTIC SEQ. ID NO: 481 CHTSCSPACQ SEQ. ID NO: 482 CHTSCSSGCQ SEQ. ID NO: 483 CHTTCYRPAC SEQ. ID NO: 484 CHTTCYRPTC SEQ. ID NO: 485 CIHSPCQASC SEQ. ID NO: 486 CIHSTHCGCN SEQ. ID NO: 487 CIRSPCQASC SEQ. ID NO: 488 CISSCYRPQC SEQ. ID NO: 489 CISSPCQQSC SEQ. ID NO: 490 CKPCSSQSSC SEQ. ID NO: 491 CKPSCSQSSC SEQ. ID NO: 492 CKPVCFKPIC SEQ. ID NO: 493 CKPVCYVPTC SEQ. ID NO: 494 CKPVSCVPVC SEQ. ID NO: 495 CKPVYCVPVC SEQ. ID NO: 496 CKTVYCKPIC SEQ. ID NO: 497 CLNQSCGSNC SEQ. ID NO: 498 CLNQSCGSSC SEQ. ID NO: 499 CLPGCLNQSC SEQ. ID NO: 500 CLPGSCDSCS SEQ. ID NO: 501 CLPPCYLVSC SEQ. ID NO: 502 CLPTSCQPSC SEQ. ID NO: 503 CLSFLCRPAC SEQ. ID NO: 504 CLVSSCQPSC SEQ. ID NO: 505 CMPSPCQPAC SEQ. ID NO: 506 CMSGSCQAAC SEQ. ID NO: 507 CNESSYCLPC SEQ. ID NO: 508 CPASCVSLLC SEQ. ID NO: 509 CPMACPGSPC SEQ. ID NO: 510 CPSSCTAVVC SEQ. ID NO: 511 CPVTCEPSPC SEQ. ID NO: 512 CQAACEPSAC SEQ. ID NO: 513 CQAACEPSPC SEQ. ID NO: 514 CQAACGQSVC SEQ. ID NO: 515 CQAPCSTKNC SEQ. ID NO: 516 CQAVCEPSPC SEQ. ID NO: 517 CQDSCGSSSC SEQ. ID NO: 518 CQHSSCQPTC SEQ. ID NO: 519 CQISSCGTGC SEQ. ID NO: 520 CQKSSCQPAC SEQ. ID NO: 521 CQPMCSHAAC SEQ. ID NO: 522 CQPPCTTALC SEQ. ID NO: 523 CQPSCESSFC SEQ. ID NO: 524 CQPSCSESTC SEQ. ID NO: 525 CQPSCTSVLC SEQ. ID NO: 526 CQPTCGGSSC SEQ. ID NO: 527 CQPTCSRPSC SEQ. ID NO: 528 CQPVCPTPTC SEQ. ID NO: 529 CQPVLCKSSC SEQ. ID NO: 530 CQPVVCEPSC SEQ. ID NO: 531 CQQPSCQPAC SEQ. ID NO: 532 CQQSCRVPVC SEQ. ID NO: 533 CQQSCYVPVC SEQ. ID NO: 534 CQQSGCQPAC SEQ. ID NO: 535 CQQSSCHPAC SEQ. ID NO: 536 CQQSSCKPAC SEQ. ID NO: 537 CQQSSCQLAC SEQ. ID NO: 538 CQQSSCQPAC SEQ. ID NO: 539 CQQSSCQPTC SEQ. ID NO: 540 CQQSSCQSAC SEQ. ID NO: 541 CQQSSCVSCV SEQ. ID NO: 542 CQQSYCVPVC SEQ. ID NO: 543 CQSGCISSCT SEQ. ID NO: 544 CQSGCTDSCT SEQ. ID NO: 545 CQSGCTSSCT SEQ. ID NO: 546 CQSSCYRPTC SEQ. ID NO: 547 CQSVCYQPTC SEQ. ID NO: 548 CQSVYCQPTC SEQ. ID NO: 549 CQTACEPSAC SEQ. ID NO: 550 CQTSSCGTGC SEQ. ID NO: 551 CQTTCHPSCG SEQ. ID NO: 552 CQTTCRPSCG SEQ. ID NO: 553 CQTTCYRTTC SEQ. ID NO: 554 CQTTRCRTTC SEQ. ID NO: 555 CQVTCEPSPC SEQ. ID NO: 556 CRNTSCQPTC SEQ. ID NO: 557 CRPACSPLAC SEQ. ID NO: 558 CRPACSRLAC SEQ. ID NO: 559 CRPACSRPAC SEQ. ID NO: 560 CRPMCSRPAC SEQ. ID NO: 561 CRPSCGQTTC SEQ. ID NO: 562 CRPSCGVSSC SEQ. ID NO: 563 CRPSCSISSC SEQ. ID NO: 564 CRPSCSQTTC SEQ. ID NO: 565 CRPSYCGQSC SEQ. ID NO: 566 CRPSYCISSC SEQ. ID NO: 567 CRPSYCQTTC SEQ. ID NO: 568 CRPTCSRLAC SEQ. ID NO: 569 CRPTCSSGSC SEQ. ID NO: 570 CRPTSCQNTC SEQ. ID NO: 571 CRPVCRSTYC SEQ. ID NO: 572 CRPVCSRPAC SEQ. ID NO: 573 CRPVTCVPRC SEQ. ID NO: 574 CRQSSCQPAC SEQ. ID NO: 575 CRTTCFHPIC SEQ. ID NO: 576 CRTTCFQPTC SEQ. ID NO: 577 CRTTCYRPSC SEQ. ID NO: 578 CRTTYCRPSC SEQ. ID NO: 579 CRVTCEPSPC SEQ. ID NO: 580 CRYGCGHRGC SEQ. ID NO: 581 CSAPCVALLC SEQ. ID NO: 582 CSDDSGSCCQ SEQ. ID NO: 583 CSEDSSSCCQ SEQ. ID NO: 584 CSEDSYSCCQ SEQ. ID NO: 585 CSEGCGSGCG SEQ. ID NO: 586 CSESSPSCCQ SEQ. ID NO: 587 CSESSSSCCQ SEQ. ID NO: 588 CSFDKSCRCG SEQ. ID NO: 589 CSGASSLCCQ SEQ. ID NO: 590 CSGASSPCCQ SEQ. ID NO: 591 CSGASSSCCQ SEQ. ID NO: 592 CSGASTSCCQ SEQ. ID NO: 593 CSGGCGSGCG SEQ. ID NO: 594 CSGGCGSSCG SEQ. ID NO: 595 CSGISSSCCQ SEQ. ID NO: 596 CSKDSSSCCQ SEQ. ID NO: 597 CSKGACGSCG SEQ. ID NO: 598 CSLSCGSRSC SEQ. ID NO: 599 CSQDLCQETC SEQ. ID NO: 600 CSRGCGSGCG SEQ. ID NO: 601 CSRLSSACCG SEQ. ID NO: 602 CSSCGKGGCG SEQ. ID NO: 603 CSSCGKRGCG SEQ. ID NO: 604 CSSDKSCRCG SEQ. ID NO: 605 CSSGNFSSCC SEQ. ID NO: 606 CSSSGCGSFC SEQ. ID NO: 607 CSSSGCGSSC SEQ. ID NO: 608 CSTPCYQPIC SEQ. ID NO: 609 CSTTCRTSSC SEQ. ID NO: 610 CSWVPACSCT SEQ. ID NO: 611 CTFSPCQQAC SEQ. ID NO: 612 CTMSVCSSAC SEQ. ID NO: 613 CTRPICEPCR SEQ. ID NO: 614 CTSSPCQHAC SEQ. ID NO: 615 CTSSPCQQAC SEQ. ID NO: 616 CTSSPCQQSC SEQ. ID NO: 617 CTSSSCQQAC SEQ. ID NO: 618 CVALLCRPLC SEQ. ID NO: 619 CVALVCEPVC SEQ. ID NO: 620 CVFSSCNTTC SEQ. ID NO: 621 CVGFVCQPMC SEQ. ID NO: 622 CVPRCTRPIC SEQ. ID NO: 623 CVPSPCQVAC SEQ. ID NO: 624 CVPSRCQASC SEQ. ID NO: 625 CVPSSCQASC SEQ. ID NO: 626 CVPVCNKPVC SEQ. ID NO: 627 CVPVCSKSVC SEQ. ID NO: 628 CVPVRCKPVC SEQ. ID NO: 629 CVSLLCRPAC SEQ. ID NO: 630 CVSLLCRPMC SEQ. ID NO: 631 CVSLLCRPTC SEQ. ID NO: 632 CVSLLCRPVC SEQ. ID NO: 633 CVSNPCQVTC SEQ. ID NO: 634 CVSRCYRPHC SEQ. ID NO: 635 CVSSCFRPQC SEQ. ID NO: 636 CVSSICQPIC SEQ. ID NO: 637 CVSSPCQPTC SEQ. ID NO: 638 CVVSCTPPSC SEQ. ID NO: 639 CVVSCTPPTC SEQ. ID NO: 640 CYCPKNSIFC SEQ. ID NO: 641 CYEPCLPRGC SEQ. ID NO: 642 CYRRCYSSCY SEQ. ID NO: 643 GCCGYGGLGC SEQ. ID NO: 644 GCGGCGSGCA SEQ. ID NO: 645 GCGGCGSGCG SEQ. ID NO: 646 GCGGCGSSCG SEQ. ID NO: 647 GCGGCSSSCG SEQ. ID NO: 648 GCGGSGSSCC SEQ. ID NO: 649 GCGSGCAGCG SEQ. ID NO: 650 GCGSGCGGCG SEQ. ID NO: 651 GCGSGCGGCS SEQ. ID NO: 652 GCGSSCGGCD SEQ. ID NO: 653 GCGSSCGGCG SEQ. ID NO: 654 GCGSSCSQCS SEQ. ID NO: 655 GCGYSSSCCG SEQ. ID NO: 656 GCKGGCGSCG SEQ. ID NO: 657 GCSGCSGGCG SEQ. ID NO: 658 ICSGASSLCC SEQ. ID NO: 659 ICSGASSPCC SEQ. ID NO: 660 MCCNYYGNSC SEQ. ID NO: 661 MCCNYYRNSC SEQ. ID NO: 662 MCYGYGCGCG SEQ. ID NO: 663 NCCSRNFSSC SEQ. ID NO: 664 PCSLQEGCCR SEQ. ID NO: 665 PCSSQSSCCV SEQ. ID NO: 666 SCCAPASSCQ SEQ. ID NO: 667 SCCAPASTCQ SEQ. ID NO: 668 SCCAPTSSCQ SEQ. ID NO: 669 SCCGYRPLCY SEQ. ID NO: 670 SCCVPASSCQ SEQ. ID NO: 671 SCCVPTSSCQ SEQ. ID NO: 672 SCGCSKGACG SEQ. ID NO: 673 SCGGCDSSCG SEQ. ID NO: 674 SCGGCGSGCG SEQ. ID NO: 675 SCGGCGSSCG SEQ. ID NO: 676 SCGGCKGGCG SEQ. ID NO: 677 SCGGSKGCCG SEQ. ID NO: 678 SCGSGCRGCG SEQ. ID NO: 679 SCYGCGYGCI SEQ. ID NO: 680 TCCVPVPSCG SEQ. ID NO: 681 TCSDDSGSCC SEQ. ID NO: 682 TCSEDSSSCC SEQ. ID NO: 683 TCSEDSYSCC SEQ. ID NO: 684 TCSESSPSCC SEQ. ID NO: 685 TCSESSSSCC SEQ. ID NO: 686 TCSKDSSSCC SEQ. ID NO: 687 TCSRLSSACC SEQ. ID NO: 688 VCCQPTPICD SEQ. ID NO: 689 VCSEDSSSCC SEQ. ID NO: 690 VCSGASSLCC SEQ. ID NO: 691 VCSGASSPCC SEQ. ID NO: 692 VCSGASSSCC SEQ. ID NO: 693 VCSGASTSCC SEQ. ID NO: 694 VCSGDSSCCQ SEQ. ID NO: 695 VCSGISSSCC SEQ. ID NO: 696 YCVPIPSCCA SEQ. ID NO: 697 CASSCCTPSC SEQ. ID NO: 698 CCDNCPPPCH SEQ. ID NO: 699 CCEPCLPRGC SEQ. ID NO: 700 CCGAASSCCR SEQ. ID NO: 701 CCGCGGSGCG SEQ. ID NO: 702 CCGPSSSCCQ SEQ. ID NO: 703 CCGSGCGGCG SEQ. ID NO: 704 CCKPYCSQCS SEQ. ID NO: 705 CCMPVSSCCA SEQ. ID NO: 706 CCNYYRNCCG SEQ. ID NO: 707 CCPSCVVSSC SEQ. ID NO: 708 CCPSYCVSSC SEQ. ID NO: 709 CCQPICGSSC SEQ. ID NO: 710 CCQPICVTSC SEQ. ID NO: 711 CCQPTCLSSC SEQ. ID NO: 712 CCQPTCLTSC SEQ. ID NO: 713 CCQPTCVASC SEQ. ID NO: 714 CCQPTCVTSC SEQ. ID NO: 715 CCQPYCHPTC SEQ. ID NO: 716 CCQQSSCVSC SEQ. ID NO: 717 CCQSSCFKPC SEQ. ID NO: 718 CCQSSCSKPC SEQ. ID NO: 719 CCQSSCYKPC SEQ. ID NO: 720 CCQTICRSTC SEQ. ID NO: 721 CCQTTCHPSC SEQ. ID NO: 722 CCQTTCRPSC SEQ. ID NO: 723 CCRVPTCSCS SEQ. ID NO: 724 CCSPGCQPTC SEQ. ID NO: 725 CCSSGCGSSC SEQ. ID NO: 726 CCSSSCGSCG SEQ. ID NO: 727 CCTQEQNCCE SEQ. ID NO: 728 CCVPIPSCCA SEQ. ID NO: 729 CCVPISSCCA SEQ. ID NO: 730 CCVPVCYQCK SEQ. ID NO: 731 CCVPVPSCCA SEQ. ID NO: 732 CCVPVPSCCV SEQ. ID NO: 733 CCVPVSSCCA SEQ. ID NO: 734 CDSSCCQPSC SEQ. ID NO: 735 CDTCPPPCCK SEQ. ID NO: 736 CEPCRRPVCC SEQ. ID NO: 737 CEPSCCQPVC SEQ. ID NO: 738 CEPSCCSAVC SEQ. ID NO: 739 CETSCCQPSC SEQ. ID NO: 740 CETTCCRTTC SEQ. ID NO: 741 CFSGCGSSCC SEQ. ID NO: 742 CGCSQSNCCK SEQ. ID NO: 743 CGCSQSSCCK SEQ. ID NO: 744 CGGCGGCGGC SEQ. ID NO: 745 CGGCGGGCCG SEQ. ID NO: 746 CGGCGSGCCV SEQ. ID NO: 747 CGGCGSSCCV SEQ. ID NO: 748 CGGGCCGSSC SEQ. ID NO: 749 CGGSCCGSSC SEQ. ID NO: 750 CGQSCCRPAC SEQ. ID NO: 751 CGQSCCRPVC SEQ. ID NO: 752 CGSCGCSQCN SEQ. ID NO: 753 CGSCGCSQCS SEQ. ID NO: 754 CGSFCCQSSC SEQ. ID NO: 755 CGSGCCVPVC SEQ. ID NO: 756 CGSSCCGSGC SEQ. ID NO: 757 CGSSCCQPCY SEQ. ID NO: 758 CGSSCCQPIC SEQ. ID NO: 759 CGSSCCQPSC SEQ. ID NO: 760 CGSSCCQSSC SEQ. ID NO: 761 CGSSCCVPIC SEQ. ID NO: 762 CGSSCCVPVC SEQ. ID NO: 763 CGSSCSQCSC SEQ. ID NO: 764 CGYGSCCGCG SEQ. ID NO: 765 CHPRCCISSC SEQ. ID NO: 766 CHPSCCESSC SEQ. ID NO: 767 CHPSCCISSC SEQ. ID NO: 768 CHPTCCQNTC SEQ. ID NO: 769 CHPTCCQTIC SEQ. ID NO: 770 CHPVCCQTTC SEQ. ID NO: 771 CHPVCKSTCC SEQ. ID NO: 772 CHPVCRSTCC SEQ. ID NO: 773 CISSCCHPSC SEQ. ID NO: 774 CISSCCKPSC SEQ. ID NO: 775 CISSCCRPSC SEQ. ID NO: 776 CISSCTPSCC SEQ. ID NO: 777 CISSSCCPSC SEQ. ID NO: 778 CKAVCCVPTC SEQ. ID NO: 779 CKPCCSQASC SEQ. ID NO: 780 CKPCCSQSRC SEQ. ID NO: 781 CKPCCSQSSC SEQ. ID NO: 782 CKPCCSSSGC SEQ. ID NO: 783 CKPCSCFSGC SEQ. ID NO: 784 CKPCSCSSGC SEQ. ID NO: 785 CKPCYCSSGC SEQ. ID NO: 786 CKPICCVPVC SEQ. ID NO: 787 CKPQCCQSVC SEQ. ID NO: 788 CKPSCCQTTC SEQ. ID NO: 789 CKPVCCAPTC SEQ. ID NO: 790 CKPVCCKPIC SEQ. ID NO: 791 CKPVCCKSIC SEQ. ID NO: 792 CKPVCCLPTC SEQ. ID NO: 793 CKPVCCVPTC SEQ. ID NO: 794 CKPVCCVPVC SEQ. ID NO: 795 CKPVCCVSTC SEQ. ID NO: 796 CKPYCCQSSC SEQ. ID NO: 797 CKPYCSQCSC SEQ. ID NO: 798 CKSNCCKPVC SEQ. ID NO: 799 CKTVCCKPVC SEQ. ID NO: 800 CLPPCCVVSC SEQ. ID NO: 801 CLTSCCQPSC SEQ. ID NO: 802 CNPCCSQSSC SEQ. ID NO: 803 CPESCCELPC SEQ. ID NO: 804 CPESCCEPHC SEQ. ID NO: 805 CPESCCEPPC SEQ. ID NO: 806 CPFSCPTTCC SEQ. ID NO: 807 CPGDCFTCCT SEQ. ID NO: 808 CPSCVVSSCC SEQ. ID NO: 809 CPSYCVSSCC SEQ. ID NO: 810 CPTTCCRTTC SEQ. ID NO: 811 CQETCCRPSC SEQ. ID NO: 812 CQHACCVPVC SEQ. ID NO: 813 CQNTCCRTTC SEQ. ID NO: 814 CQPACCQPTC SEQ. ID NO: 815 CQPACCTASC SEQ. ID NO: 816 CQPACCTSSC SEQ. ID NO: 817 CQPACCTTSC SEQ. ID NO: 818 CQPACCVPVC SEQ. ID NO: 819 CQPACCVSSC SEQ. ID NO: 820 CQPCCHPTCY SEQ. ID NO: 821 CQPCCRPTSC SEQ. ID NO: 822 CQPICCGSSC SEQ. ID NO: 823 CQPICGSSCC SEQ. ID NO: 824 CQPICVTSCC SEQ. ID NO: 825 CQPNCCRPSC SEQ. ID NO: 826 CQPRCCETSC SEQ. ID NO: 827 CQPSCCRPAC SEQ. ID NO: 828 CQPSCCSTPC SEQ. ID NO: 829 CQPSCCSTTC SEQ. ID NO: 830 CQPSCCVPSC SEQ. ID NO: 831 CQPSCCVSSC SEQ. ID NO: 832 CQPTCCGSSC SEQ. ID NO: 833 CQPTCCHPSC SEQ. ID NO: 834 CQPTCCQPTC SEQ. ID NO: 835 CQPTCCRPRC SEQ. ID NO: 836 CQPTCCRPSC SEQ. ID NO: 837 CQPTCCRTTC SEQ. ID NO: 838 CQPTCLSSCC SEQ. ID NO: 839 CQPTCLTSCC SEQ. ID NO: 840 CQPTCVASCC SEQ. ID NO: 841 CQPTCVTSCC SEQ. ID NO: 842 CQPVCCQPTC SEQ. ID NO: 843 CQPYCHPTCC SEQ. ID NO: 844 CQQACCMPVC SEQ. ID NO: 845 CQQACCVPIC SEQ. ID NO: 846 CQQACCVPVC SEQ. ID NO: 847 CQQSCCVPVC SEQ. ID NO: 848 CQQSCCVSVC SEQ. ID NO: 849 CQSMCCQPTC SEQ. ID NO: 850 CQSNCCVPVC SEQ. ID NO: 851 CQSSCCKPCS SEQ. ID NO: 852 CQSSCCQSSC SEQ. ID NO: 853 CQSSCCVPVC SEQ. ID NO: 854 CQSSCFKPCC SEQ. ID NO: 855 CQSSCSKPCC SEQ. ID NO: 856 CQSVCCQPTC SEQ. ID NO: 857 CQTICRSTCC SEQ. ID NO: 858 CQTTCCRPSC SEQ. ID NO: 859 CQTTCCRTTC SEQ. ID NO: 860 CRATCCRPSC SEQ. ID NO: 861 CRGCGPSCCA SEQ. ID NO: 862 CRPACCETTC SEQ. ID NO: 863 CRPACCQNTC SEQ. ID NO: 864 CRPCCWATTC SEQ. ID NO: 865 CRPICRPACC SEQ. ID NO: 866 CRPLCCQTTC SEQ. ID NO: 867 CRPQCCQSVC SEQ. ID NO: 868 CRPQCCQTTC SEQ. ID NO: 869 CRPRCCISSC SEQ. ID NO: 870 CRPSCCESSC SEQ. ID NO: 871 CRPSCCETTC SEQ. ID NO: 872 CRPSCCISSC SEQ. ID NO: 873 CRPSCCKPQC SEQ. ID NO: 874 CRPSCCMSSC SEQ. ID NO: 875 CRPSCCQTTC SEQ. ID NO: 876 CRPSCCRPSC SEQ. ID NO: 877 CRPSCCVSRC SEQ. ID NO: 878 CRPSCCVSSC SEQ. ID NO: 879 CRPTCCETTC SEQ. ID NO: 880 CRPTCCQNTC SEQ. ID NO: 881 CRPTCCQTTC SEQ. ID NO: 882 CRPVCCDPCS SEQ. ID NO: 883 CRPVCCQTTC SEQ. ID NO: 884 CRPVCQPACC SEQ. ID NO: 885 CRPVCRPACC SEQ. ID NO: 886 CRPVCRPTCC SEQ. ID NO: 887 CRPVCRSTCC SEQ. ID NO: 888 CRPYCCESSC SEQ. ID NO: 889 CRRPVCCDPC SEQ. ID NO: 890 CRSQCCQSVC SEQ. ID NO: 891 CRTTCCHPSC SEQ. ID NO: 892 CRTTCCQPIC SEQ. ID NO: 893 CRTTCCQPTC SEQ. ID NO: 894 CRTTCCRPSC SEQ. ID NO: 895 CRTTCCRTTC SEQ. ID NO: 896 CSCSSCGSCA SEQ. ID NO: 897 CSCSSCGSCG SEQ. ID NO: 898 CSCTSCGSCG SEQ. ID NO: 899 CSPACQPTCC SEQ. ID NO: 900 CSPGCQPTCC SEQ. ID NO: 901 CSPSCCQTTC SEQ. ID NO: 902 CSQCSCYKPC SEQ. ID NO: 903 CSQSNCCKPC SEQ. ID NO: 904 CSQSSCCKPC SEQ. ID NO: 905 CSSGCGSCCQ SEQ. ID NO: 906 CSSGCGSSCC SEQ. ID NO: 907 CSSGCQPACC SEQ. ID NO: 908 CSSSCCQPSC SEQ. ID NO: 909 CSTPCCQPTC SEQ. ID NO: 910 CSTTCCQPIC SEQ. ID NO: 911 CTAVVCRPCC SEQ. ID NO: 912 CTDSCTPSCC SEQ. ID NO: 913 CTPSCCQPAC SEQ. ID NO: 914 CTRPICEPCC SEQ. ID NO: 915 CTSSCTPSCC SEQ. ID NO: 916 CVPACSCSSC SEQ. ID NO: 917 CVPACSCTSC SEQ. ID NO: 918 CVPVCCKPVC SEQ. ID NO: 919 CVPVCCVPTC SEQ. ID NO: 920 CVPVCCVPVC SEQ. ID NO: 921 CVSCVSSPCC SEQ. ID NO: 922 CVSRCCRPQC SEQ. ID NO: 923 CVSSCCKPQC SEQ. ID NO: 924 CVSSCCQHSC SEQ. ID NO: 925 CVSSCCQPFC SEQ. ID NO: 926 CVSSCCQPSC SEQ. ID NO: 927 CVSSCCRPQC SEQ. ID NO: 928 CVSTCCRPTC SEQ. ID NO: 929 CVTRCCSTPC SEQ. ID NO: 930 CVTSCCQPAC SEQ. ID NO: 931 CVTSCCQPSC SEQ. ID NO: 932 CVYSCCQPFC SEQ. ID NO: 933 CVYSCCQPSC SEQ. ID NO: 934 GCCGCSEGCG SEQ. ID NO: 935 GCCGCSGGCG SEQ. ID NO: 936 GCCGCSRGCG SEQ. ID NO: 937 GCCRPITCCP SEQ. ID NO: 938 GCGSSCCQCS SEQ. ID NO: 939 GCGVPVCCCS SEQ. ID NO: 940 LCCPCQTTCS SEQ. ID NO: 941 PCCCLRPVCG SEQ. ID NO: 942 PCCCRPVTCQ SEQ. ID NO: 943 PCCCVRPVCG SEQ. ID NO: 944 PCCSQASCCV SEQ. ID NO: 945 PCCSQSRCCV SEQ. ID NO: 946 PCCSQSSCCK SEQ. ID NO: 947 PCCSQSSCCV SEQ. ID NO: 948 PCCWATTCCQ SEQ. ID NO: 949 QCSCCKPYCS SEQ. ID NO: 950 RCYVPVCCCK SEQ. ID NO: 951 SCCAPVYCCK SEQ. ID NO: 952 SCCISSSCCP SEQ. ID NO: 953 SCCVSSCRCP SEQ. ID NO: 954 SCGCSQCSCY SEQ. ID NO: 955 SCGLENCCCP SEQ. ID NO: 956 VCCGASSCCQ SEQ. ID NO: 957 VCCGDSSCCQ SEQ. ID NO: 958 CASSCCTPSCC SEQ. ID NO: 959 CCCPSCVVSSC SEQ. ID NO: 960 CCCPSYCVSSC SEQ. ID NO: 961 CCCSSGCGSSC SEQ. ID NO: 962 CCDTCPPPCCK SEQ. ID NO: 963 CCEPHCCALSC SEQ. ID NO: 964 CCEPPCCAPSC SEQ. ID NO: 965 CCEPPCCATSC SEQ. ID NO: 966 CCETSCCQPSC SEQ. ID NO: 967 CCGSSCCGSGC SEQ. ID NO: 968 CCGSSCCGSSC SEQ. ID NO: 969 CCHPRCCISSC SEQ. ID NO: 970 CCHPSCCESSC SEQ. ID NO: 971 CCHPSCCISSC SEQ. ID NO: 972 CCHPSCCVSSC SEQ. ID NO: 973 CCHPTCCQNTC SEQ. ID NO: 974 CCHPTCCQTIC SEQ. ID NO: 975 CCISSCCKPSC SEQ. ID NO: 976 CCISSCCRPSC SEQ. ID NO: 977 CCISSSCCPSC SEQ. ID NO: 978 CCKAVCCVPTC SEQ. ID NO: 979 CCKPCCSQASC SEQ. ID NO: 980 CCKPCCSQSRC SEQ. ID NO: 981 CCKPCCSQSSC SEQ. ID NO: 982 CCKPCCSSSGC SEQ. ID NO: 983 CCKPCSCFSGC SEQ. ID NO: 984 CCKPCSCSSGC SEQ. ID NO: 985 CCKPCYCSSGC SEQ. ID NO: 986 CCKPICCVPVC SEQ. ID NO: 987 CCKPQCCQSVC SEQ. ID NO: 988 CCKPVCCKPIC SEQ. ID NO: 989 CCKPYCCQSSC SEQ. ID NO: 990 CCKPYCSQCSC SEQ. ID NO: 991 CCMPVCCKPVC SEQ. ID NO: 992 CCMPVCCKTVC SEQ. ID NO: 993 CCMSSCCKPQC SEQ. ID NO: 994 CCNPCCSQSSC SEQ. ID NO: 995 CCPGDCFTCCT SEQ. ID NO: 996 CCPSCVVSSCC SEQ. ID NO: 997 CCPSYCVSSCC SEQ. ID NO: 998 CCQNTCCRTTC SEQ. ID NO: 999 CCQPACCVSSC SEQ. ID NO: 1000 CCQPCCHPTCY SEQ. ID NO: 1001 CCQPCCRPTSC SEQ. ID NO: 1002 CCQPICGSSCC SEQ. ID NO: 1003 CCQPICVTSCC SEQ. ID NO: 1004 CCQPNCCRPSC SEQ. ID NO: 1005 CCQPSCCETSC SEQ. ID NO: 1006 CCQPSCCRPAC SEQ. ID NO: 1007 CCQPSCCSTPC SEQ. ID NO: 1008 CCQPSCCSTTC SEQ. ID NO: 1009 CCQPSCCVPSC SEQ. ID NO: 1010 CCQPSCCVSSC SEQ. ID NO: 1011 CCQPTCCHPSC SEQ. ID NO: 1012 CCQPTCCQPTC SEQ. ID NO: 1013 CCQPTCCRPRC SEQ. ID NO: 1014 CCQPTCCRPSC SEQ. ID NO: 1015 CCQPTCCRPTC SEQ. ID NO: 1016 CCQPTCCRTTC SEQ. ID NO: 1017 CCQPTCLSSCC SEQ. ID NO: 1018 CCQPTCLTSCC SEQ. ID NO: 1019 CCQPTCVASCC SEQ. ID NO: 1020 CCQPTCVTSCC SEQ. ID NO: 1021 CCQPYCHPTCC SEQ. ID NO: 1022 CCQSMCCQPTC SEQ. ID NO: 1023 CCQSNCCVPVC SEQ. ID NO: 1024 CCQSSCCKPCS SEQ. ID NO: 1025 CCQSSCCKPSC SEQ. ID NO: 1026 CCQSSCCKPYC SEQ. ID NO: 1027 CCQSSCCQSSC SEQ. ID NO: 1028 CCQSSCCVPVC SEQ. ID NO: 1029 CCQSSCFKPCC SEQ. ID NO: 1030 CCQSSCSKPCC SEQ. ID NO: 1031 CCQSSCYKPCC SEQ. ID NO: 1032 CCQSVCCQPTC SEQ. ID NO: 1033 CCQTICRSTCC SEQ. ID NO: 1034 CCQTTCCRPSC SEQ. ID NO: 1035 CCQTTCCRTTC SEQ. ID NO: 1036 CCRPACCETTC SEQ. ID NO: 1037 CCRPACCQNTC SEQ. ID NO: 1038 CCRPLCCQTTC SEQ. ID NO: 1039 CCRPQCCQSVC SEQ. ID NO: 1040 CCRPQCCQTTC SEQ. ID NO: 1041 CCRPSCCESSC SEQ. ID NO: 1042 CCRPSCCETTC SEQ. ID NO: 1043 CCRPSCCGSSC SEQ. ID NO: 1044 CCRPSCCISSC SEQ. ID NO: 1045 CCRPSCCKPQC SEQ. ID NO: 1046 CCRPSCCQTTC SEQ. ID NO: 1047 CCRPSCCVSRC SEQ. ID NO: 1048 CCRPSCCVSSC SEQ. ID NO: 1049 CCRPTCCQNTC SEQ. ID NO: 1050 CCRPTCCQTTC SEQ. ID NO: 1051 CCRPVCCDPCS SEQ. ID NO: 1052 CCRTTCCQPTC SEQ. ID NO: 1053 CCRTTCCRPSC SEQ. ID NO: 1054 CCRTTCCRTTC SEQ. ID NO: 1055 CCSCSSCGSCA SEQ. ID NO: 1056 CCSPGCQPTCC SEQ. ID NO: 1057 CCSQSSCCKPC SEQ. ID NO: 1058 CCSSGCGSCCQ SEQ. ID NO: 1059 CCSSGCGSSCC SEQ. ID NO: 1060 CCSTPCCQPTC SEQ. ID NO: 1061 CCVPACSCSSC SEQ. ID NO: 1062 CCVPACSCTSC SEQ. ID NO: 1063 CCVPICCKPIC SEQ. ID NO: 1064 CCVPICCKPVC SEQ. ID NO: 1065 CCVPVCCKPIC SEQ. ID NO: 1066 CCVPVCCKPVC SEQ. ID NO: 1067 CCVPVCCKSNC SEQ. ID NO: 1068 CCVPVCCKTVC SEQ. ID NO: 1069 CCVPVCCSSSC SEQ. ID NO: 1070 CCVPVCCVPVC SEQ. ID NO: 1071 CCVSSCCKPQC SEQ. ID NO: 1072 CCVSSCCQHSC SEQ. ID NO: 1073 CCVSSCCQPSC SEQ. ID NO: 1074 CCVSSCCRPQC SEQ. ID NO: 1075 CCVSTCCRPTC SEQ. ID NO: 1076 CCVSVCCKPVC SEQ. ID NO: 1077 CDSSCCQPSCC SEQ. ID NO: 1078 CEPCCRPVCCD SEQ. ID NO: 1079 CFKPCCCQSSC SEQ. ID NO: 1080 CGDGCCCPSCY SEQ. ID NO: 1081 CGGGCCGSSCC SEQ. ID NO: 1082 CGGSCCGSSCC SEQ. ID NO: 1083 CGLENCCCPSC SEQ. ID NO: 1084 CGQSCCRPACC SEQ. ID NO: 1085 CGQSCCRPVCC SEQ. ID NO: 1086 CGSCCQSSCCN SEQ. ID NO: 1087 CGSCGCSQCNC SEQ. ID NO: 1088 CGSCGCSQCSC SEQ. ID NO: 1089 CGSGCCGPVCC SEQ. ID NO: 1090 CGSGCCVPVCC SEQ. ID NO: 1091 CGSNCCQPCCR SEQ. ID NO: 1092 CGSSCCQPCCH SEQ. ID NO: 1093 CGSSCCQPCCR SEQ. ID NO: 1094 CGSSCCQPCYC SEQ. ID NO: 1095 CGSSCCQPSCC SEQ. ID NO: 1096 CGSSCCQSSCC SEQ. ID NO: 1097 CGSSCCVPICC SEQ. ID NO: 1098 CGSSCCVPVCC SEQ. ID NO: 1099 CGSSCSQCSCC SEQ. ID NO: 1100 CGVPVCCCSCS SEQ. ID NO: 1101 CHPRCCISSCC SEQ. ID NO: 1102 CHPSCCESSCC SEQ. ID NO: 1103 CHPSCCISSCC SEQ. ID NO: 1104 CHPTCCQNTCC SEQ. ID NO: 1105 CISSCCHPSCC SEQ. ID NO: 1106 CISSCCKPSCC SEQ. ID NO: 1107 CISSCCRPSCC SEQ. ID NO: 1108 CISSSCCPSCC SEQ. ID NO: 1109 CKPCCCSSGCG SEQ. ID NO: 1110 CKPCCSQASCC SEQ. ID NO: 1111 CKPCCSQSRCC SEQ. ID NO: 1112 CKPCCSQSSCC SEQ. ID NO: 1113 CKPQCCQSMCC SEQ. ID NO: 1114 CKPQCCQSVCC SEQ. ID NO: 1115 CKPVCCCVPAC SEQ. ID NO: 1116 CKPVCCKPICC SEQ. ID NO: 1117 CKPVCCMPVCC SEQ. ID NO: 1118 CKPVCCVPVCC SEQ. ID NO: 1119 CKPVCCVSVCC SEQ. ID NO: 1120 CKPYCSQCSCC SEQ. ID NO: 1121 CLPCCRPTCCQ SEQ. ID NO: 1122 CLTSCCQPSCC SEQ. ID NO: 1123 CMSSCCKPQCC SEQ. ID NO: 1124 CNPCCSQSSCC SEQ. ID NO: 1125 CPACCVSSCCQ SEQ. ID NO: 1126 CPESCCEPHCC SEQ. ID NO: 1127 CPESCCEPPCC SEQ. ID NO: 1128 CPSCCESSCCR SEQ. ID NO: 1129 CPSCCQTTCCR SEQ. ID NO: 1130 CPSCCVSSCCR SEQ. ID NO: 1131 CQCSCCKPYCS SEQ. ID NO: 1132 CQETCCRPSCC SEQ. ID NO: 1133 CQNTCCRTTCC SEQ. ID NO: 1134 CQPACCTASCC SEQ. ID NO: 1135 CQPACCTSSCC SEQ. ID NO: 1136 CQPACCTTSCC SEQ. ID NO: 1137 CQPACCVPVCC SEQ. ID NO: 1138 CQPACCVSSCC SEQ. ID NO: 1139 CQPCCHPTCCQ SEQ. ID NO: 1140 CQPCCRPACCE SEQ. ID NO: 1141 CQPCCRPACCQ SEQ. ID NO: 1142 CQPCCRPTCCQ SEQ. ID NO: 1143 CQPCYCPACCV SEQ. ID NO: 1144 CQPICCGSSCC SEQ. ID NO: 1145 CQPRCCETSCC SEQ. ID NO: 1146 CQPSCCETSCC SEQ. ID NO: 1147 CQPSCCRPACC SEQ. ID NO: 1148 CQPSCCVPSCC SEQ. ID NO: 1149 CQPSCCVSSCC SEQ. ID NO: 1150 CQPTCCCPSYC SEQ. ID NO: 1151 CQPTCCGSSCC SEQ. ID NO: 1152 CQPTCCHPSCC SEQ. ID NO: 1153 CQPTCCQPTCC SEQ. ID NO: 1154 CQPTCCRPSCC SEQ. ID NO: 1155 CQPTCCRPTCC SEQ. ID NO: 1156 CQPTCCRTTCC SEQ. ID NO: 1157 CQQACCMPVCC SEQ. ID NO: 1158 CQQACCVPICC SEQ. ID NO: 1159 CQQACCVPVCC SEQ. ID NO: 1160 CQQSCCVPVCC SEQ. ID NO: 1161 CQQSCCVSVCC SEQ. ID NO: 1162 CQSNCCVPVCC SEQ. ID NO: 1163 CQSSCCCPASC SEQ. ID NO: 1164 CQSSCCKPCCS SEQ. ID NO: 1165 CQSSCCKPCSC SEQ. ID NO: 1166 CQSSCCKPYCC SEQ. ID NO: 1167 CQSSCCNPCCS SEQ. ID NO: 1168 CQSSCCQSSCC SEQ. ID NO: 1169 CQSSCCVPVCC SEQ. ID NO: 1170 CQSSCFKPCCC SEQ. ID NO: 1171 CQSSCSKPCCC SEQ. ID NO: 1172 CQSSCYKPCCC SEQ. ID NO: 1173 CQSVCCQPTCC SEQ. ID NO: 1174 CQTTCCCPSCV SEQ. ID NO: 1175 CQTTCCRPSCC SEQ. ID NO: 1176 CQTTCCRTTCC SEQ. ID NO: 1177 CRPACCETTCC SEQ. ID NO: 1178 CRPACCQNTCC SEQ. ID NO: 1179 CRPCCCLRPVC SEQ. ID NO: 1180 CRPCCCVRPVC SEQ. ID NO: 1181 CRPCCWATTCC SEQ. ID NO: 1182 CRPLCCQTTCC SEQ. ID NO: 1183 CRPQCCQSVCC SEQ. ID NO: 1184 CRPQCCQTTCC SEQ. ID NO: 1185 CRPRCCISSCC SEQ. ID NO: 1186 CRPSCCESSCC SEQ. ID NO: 1187 CRPSCCISSCC SEQ. ID NO: 1188 CRPSCCKPQCC SEQ. ID NO: 1189 CRPSCCPSCCQ SEQ. ID NO: 1190 CRPSCCQTTCC SEQ. ID NO: 1191 CRPSCCRPQCC SEQ. ID NO: 1192 CRPSCCVSRCC SEQ. ID NO: 1193 CRPSCCVSSCC SEQ. ID NO: 1194 CRPTCCQNTCC SEQ. ID NO: 1195 CRPVCCCEPTC SEQ. ID NO: 1196 CRPVCCCYSCE SEQ. ID NO: 1197 CRTTCCHPSCC SEQ. ID NO: 1198 CRTTCCRPSCC SEQ. ID NO: 1199 CSCCKPYCSQC SEQ. ID NO: 1200 CSKPCCCQSSC SEQ. ID NO: 1201 CSPCCQPTCCR SEQ. ID NO: 1202 CSPCCVSSCCQ SEQ. ID NO: 1203 CSQCSCCKPCY SEQ. ID NO: 1204 CSQCSCYKPCC SEQ. ID NO: 1205 CSQSNCCKPCC SEQ. ID NO: 1206 CSQSSCCKPCC SEQ. ID NO: 1207 CSSSCCQPSCC SEQ. ID NO: 1208 CTPSCCQPACC SEQ. ID NO: 1209 CVASCCQPSCC SEQ. ID NO: 1210 CVPICCCKPVC SEQ. ID NO: 1211 CVPSCCQPCCH SEQ. ID NO: 1212 CVPVCCCKPMC SEQ. ID NO: 1213 CVPVCCCKPVC SEQ. ID NO: 1214 CVPVCCKPVCC SEQ. ID NO: 1215 CVSSCCKPQCC SEQ. ID NO: 1216 CVSSCCQHSCC SEQ. ID NO: 1217 CVSSCCQPCCH SEQ. ID NO: 1218 CVSSCCQPCCR SEQ. ID NO: 1219 CVSSCCQPFCC SEQ. ID NO: 1220 CVSSCCQPSCC SEQ. ID NO: 1221 CVSSCCRPQCC SEQ. ID NO: 1222 CVTRCCSTPCC SEQ. ID NO: 1223 CVTSCCQPACC SEQ. ID NO: 1224 CVTSCCQPSCC SEQ. ID NO: 1225 CVYSCCQPFCC SEQ. ID NO: 1226 CVYSCCQPSCC SEQ. ID NO: 1227 CYCPACCVSSC SEQ. ID NO: 1228 CYKPCCCQSSC SEQ. ID NO: 1229 CYKPCCCSSGC SEQ. ID NO: 1230 MCCCVPACSCS SEQ. ID NO: 1231 NCCVPVCCQCK SEQ. ID NO: 1232 QCSCCKPCYCS SEQ. ID NO: 1233 QCSCYKPCCCS SEQ. ID NO: 1234 SCCVPICCQCK SEQ. ID NO: 1235 SCCVPVCCQCK SEQ. ID NO: 1236 SCGCSQCNCCK SEQ. ID NO: 1237 SCGCSQCSCCK SEQ. ID NO: 1238 VCCCVPACSCS SEQ. ID NO: 1239 VCCCVPACSCT

The present invention is of course in any way restricted to the embodiments herein described and one with ordinary skill in the area can provide many possibilities to modifications and substitutions of technical characteristics by equivalent ones, depending on each situation, as defined in the claims.

The preferred embodiments described above may obviously be combined. The following claims define further preferred embodiments. 

1. Peptide composition for hair treatment comprising at least one peptide with a sequence length of 6-12 amino acids, where 2-5 of those amino acids are cysteines, and dermatologically adequate excipients.
 2. Composition according to the previous claim wherein said peptide has a sequence length of 10-11 amino acids.
 3. Composition according to the previous claim wherein said peptide containing 3-5 cysteines.
 4. Composition according to the previous claims wherein the referred peptides comprise a percentage of hydrophobic amino acid(s) not higher than 60%, preferably less than 41%.
 5. Composition according to any of the previous claims comprising at least one hydrophobic amino acid selected from the following list: phenylalanine, alanine, leucine, methionine, isoleucine, tryptophan, proline, valine or any mixture thereof.
 6. Composition according to any of the previous claims comprising 10% to 50% of cysteine in the total of amino acids of the peptide sequence.
 7. Composition according to any of the previous claims wherein the peptide comprise at least one sequence of the following list with a degree of homology equal or higher than 90%: SEQ.ID NO: 1-SEQ.ID NO: 1239, or mixture thereof.
 8. Composition according to the previous claim wherein the peptide comprise at least one of the sequences of the following list: SEQ.ID NO: 1-SEQ.ID NO: 1239, or mixture thereof.
 9. Composition according to any of the claims 1-8 wherein the peptides comprise at least one of the sequences of the following list with a degree of homology equal or higher than 90%: SEQ.ID NO:5, SEQ.ID NO:75; SEQ.ID NO:94; SEQ.ID NO:409; SEQ.ID NO:411; SEQ.ID NO:412; SEQ ID. NO:432; SEQ.ID NO:618; SEQ.ID NO:717; SEQ.ID NO:951; SEQ.ID NO:1088; SEQ.ID NO:1131; SEQ.ID NO:1149.
 10. Composition according to the previous claim wherein peptides comprise at least one of the sequences of the following list: SEQ.ID NO:5, SEQ.ID NO:75; SEQ.ID NO:94; SEQ.ID NO:409; SEQ.ID NO:411; SEQ.ID NO:412; SEQ ID. NO:432; SEQ.ID NO:618; SEQ.ID NO:717; SEQ.ID NO:951; SEQ.ID NO:1088; SEQ.ID NO:1131; SEQ.ID NO:1149.
 11. Composition according to the previous claims characterized by the concentration of peptide vary between 0.001%-20% (w/w), and preferably 0.01-5% (w/w).
 12. Composition according to the previous claims comprising at least one of the excipients selected from the following list: surfactants, emulsifiers, preservatives, thickeners, organic polymers, humectants, silicones, oils, fragrances, vitamins, buffers, antimicrobial agents, antibacterial agents, disinfectants agents, or any mixture thereof.
 13. Composition according to the previous claims comprising at least one of the excipients selected from the following list: anionic surfactant, amphoteric surfactant, cationic surfactant, non-ionic surfactant, emulsifier, preservative, thickener, natural polymer derived, humectant, silicone, organic oil, protein, fragrance, vitamin, emollient ester, alkanolamide, amine, buffer, pH adjustor, salt, aliphatic alcohol, UV filter, amine oxide, chelate, fatty acid, antimicrobial/antibacterial agent, PEG material, polymer, anti-static agent, alcohol, or any mixture thereof.
 14. Composition according to the previous claims comprising at least one surfactant selected from the following list: anionic surfactant, amphoteric surfactant, cationic surfactant, non-ionic surfactant.
 15. Composition according to the previous claims comprising at least one compound selected from the following list: emulsifier, preservative, thickener, natural polymer derived, humectant, silicone, organic oil, protein, fragrance, vitamin, emollient ester, alkanolamide, amine, buffer, pH adjustor, salt, aliphatic alcohol, UV filter, amine oxide, chelate, fatty acid, antimicrobial agent, antibacterial agent, PEG material, polymer, anti-static agent, alcohol, or any mixture thereof.
 16. Composition according to any of the previous claims comprising fragrance, oil or any mixture thereof.
 17. Composition according to any of the previous claims comprising a dying agent/dye linked to the N or C terminal of the referred peptides.
 18. Composition according to any of the previous claims for use in medicine and/or veterinary.
 19. Composition according to any of the previous claims for use in hair treatment.
 20. Composition according to any of the previous claims for treatment of hair scalp diseases, particularly scalp irritation, alopecia areata, lichen planus, folliculitis keloid of the neck, trichorrhexis nodosa, tricodistrophy, pili torti, tricorrexis invaginata, moniletrix, uncombable hair syndrome.
 21. Use of the composition described in any of the previous claims in cosmetics.
 22. Use of the composition described in any of the previous claims for use as hair strengthener of human hair or of animal hair.
 23. Use of the composition described in any of the previous claims as hair curl or uncurl agent.
 24. Use of the composition described in any of the previous claims for hair coloring.
 25. Shampoo, lotion, serum, cream, conditioner, foam, elixir, oil, aerosol or mask characterized by comprising a composition described in any of the previous claims. 